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Oxidative stress upregulates the transcription of genes involved in Thiamine metabolism

Thiamine is a major vitamin that acts as a cofactor in energy metabolism in all organisms, as well as in lipid and amino acid metabolisms, and is associated with many diseases. It is known that glucose starvation decreases the intracellular thiamine pool while increasing oxidative stress tolerance. | Turkish Journal of Biology Turk J Biol (2018) 42: 447-452 © TÜBİTAK doi:10.3906/biy-1801-51 http://journals.tubitak.gov.tr/biology/ Research Article Oxidative Stress Upregulates the Transcription of Genes Involved in Thiamine Metabolism 1,2 1 3, Burcu KARTAL , Ahmet AKÇAY , Bedia PALABIYIK * Department of Molecular Biology and Genetics, Institute of Graduate Studies in Science and Engineering, İstanbul University, İstanbul, Turkey 2 Department of Genetics and Bioengineering, Faculty of Engineering, Alanya Alaaddin Keykubat University, Antalya, Turkey 3 Department of Molecular Biology and Genetics, Faculty of Science, İstanbul University, İstanbul, Turkey 1 Received: 24.01.2018 Accepted/Published Online: 06.08.2018 Final Version: 25.10.2018 Abstract: Thiamine is a major vitamin that acts as a cofactor in energy metabolism in all organisms, as well as in lipid and amino acid metabolisms, and is associated with many diseases. It is known that glucose starvation decreases the intracellular thiamine pool while increasing oxidative stress tolerance. Earlier, in whole genome analysis, we detected major differences in the expression of genes related to thiamine pathway against oxidative stress in Schizosaccharomyces pombe. We investigated the effects of oxidative stress and glucose repression to thiamine pathway in S. pombe by comparing some genes encoding key enzymes of each related pathway at the transcription level. In the present study, we found that the expression of genes related to thiamine biosynthesis and transport (thi2, thi3, and pho1) increased in wild type and ird11 cells grown in thiamine-rich media under oxidative stress induced by H2O2. Based on our findings, we suggested that there might be an important effect of oxidative stress on thiamine biosynthesis and transport. Key words: Fission yeast, thiamine, oxidative stress, glucose metabolism 1. Introduction Thiamine diphosphate, the biological active form of thiamine (vitamin B1), is a .