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Sử dụng tế bào nấm men cố định trong quá trình lên men Alcohol từ rỉ đường mía

Nghiên cứu này tập trung vào hiện trạng lên men rượu bao gồm tài nguyên sinh khối, nồng độ alginate khác nhau cho Saccharomyces cerevisiae cố định và tìm ra nồng độ alginate tối ưu có hại nhất lên men, sản lượng cố định tế bào, so sánh sản lượng lên men của rượu miễn phí và S.cerevisiae cố định mà các điều kiện thí nghiệm tương tự miễn phí và S.cerevisiae cố định như pH tối ưu, nhiệt độ tối ưu, nồng độ đường mật, lưu trữ ổn định, tái sử dụng và quá trình lên men hàng loạt của S.cerevisiae cố định. | Science & Technology Development, Vol 11, No.09 - 2008 USE OF IMMOBILIZED YEAST CELL IN ALCOHOL FERMENTATION FROM MOLASSES Mai Ngoc Dung, Dong Thi Thanh Thu University of Natural Sciences, VNU-HCM (Manuscript Received on January 23rd, 2007, Manuscript Revised Febuary 18th, 2008) ABSTRACT: This research focuses on the current status of alcoholic fermentation including biomass resource, various concentration of alginate for Saccharomyces cerevisiae immobilization and finding out the optimal alginate concentration that is the most alcoholic fermentation, cell immobilized yield, comparing alcoholic fermentative yield of free and immobilized S.cerevisiae which experimental conditions are the analogy of free and immobilized S.cerevisiae such as optimal pH, optimal temperature, molasses concentration, storage stability, re-use and the batch fermentation of immobilized S.cerevisiae. The results attained in this experiment indicate that the selected S.cerevisiae and entrapping method for immobilization present high and stable. Immobilized cell yield is 99.65%, fermentative yield is 68.68%, storage stability is 35 days that Alco3.0% (yeast cells were immobilized by alginate solution 3% w/w that is optimal concentration) was compared by free yeast, and re-use is 6 times for 18 days. Key words: immobilized yeast, free yeast, entrapping method, alginate. 1.INTRODUCTION The various methods are going to use cell immobilization that employing the use of immobilized yeast cells selected by entrapping method. The carrier materials of yeast immobilization include collagen, chitosan/chitin, alginate, k-carrageenan etc entrapping method. Of these, the calcium alginate is preferred because of its high fermentable activity, simple manner of preparation, and stability. [4] Alginates are linear unbranched polymers of polysaccharides family containing two uronic acid polymers but they are soluble in water. The first, that is D-mannuronic acid (M) and the second, that is L-glucuronic (G)