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Bacteriocin typing of some Turkish isolates of Pseudomonas syringae pv. phaseolicola

Eighty-six Pseudomonas syringae pv. phaseolicola isolates collected from different bean growing areas in Eskişehir were typed for the production of bacteriocin. All the isolates tested produced bacteriocin and 24 bacteriosin groups were determined. | Turk J Biol 24 (2000) 795–801 © TÜBİTAK Bacteriocin Typing of Some Turkish Isolates of Pseudomonas syringae pv. phaseolicola Kıymet GÜVEN Anadolu University, Faculty of Science, Department of Biology, 26470 Eskişehir-TURKEY Received: 11.02.1999 Abstract: Eighty-six Pseudomonas syringae pv. phaseolicola isolates collected from different bean growing areas in Eskişehir were typed for the production of bacteriocin.All the isolates tested produced bacteriocin and 24 bacteriosin groups were determined. No correlation was found between the bacteriocin groups and geographical origin. Authentic isolates of the bacterium representing 3 different races were also tested for bacteriocin production and bacteriocin types did not correlate with the races. Key Words: Pseudomonas syringae pv. phaseolicola, bacteriocin, bacteriocin typing. Türkiye Orijinli Bazı Pseudomonas syringae pv. phaseolicola İzolatlarının Bakteriosin Tiplendirmesi Özet: Eskişehir’de çeşitli fasulye üretim alanlarından toplanan 86 Pseudomonas syringae pv. phaselolicola izolatı, bakteriyosin üretme yeteneğine göre tiplendirilmiştir. Test edilen tüm izolatlar bakteriosin üretmiş ve 24 bakteriosin grubu tespit edilmiştir. Bateriosin grupları ve strainlerin coğrafik orijinleri arasında bir ilişki bulunmamıştır. Bakterinin 3 farklı ırkını temsil eden tanınmış izolatları da bakteriosin üretim için test edilmiş ve bakteriosin grupları ile ırklar arasında da bir ilişki gözlenmemiştir. Anahtar Sözcükler: Pseudomonas syringae pv. phaseolicola, bateriosin, bakteriosin tiplendirmesi. Introduction Bacteriocins are non-replicating, proteinaceous antibiotics with specificity generally restricted to closely related bacterial strains and species. Small amounts of bacteriocins are produced spontaneously in cultures of bacteriocinogenic strains, but the substances can also be induced by trating cells with ultraviolet light or Mitomycin C. Because becteriocins specifically kill the cells of sensitive bacteria, bacterial .