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Developing auto - inducible Pgrac57 promoter in Bacillus subtilis

These vectors are totally able to express GFP protein without any inducer in B. subtilis strain 1012. Moreover, GFP expression level reached 11% of the total cellular proteins. Additionally, GFP activities are in the same range with the inducer-free vector. | TRƯỜNG ĐẠI HỌC SƯ PHẠM TP HỒ CHÍ MINH HO CHI MINH CITY UNIVERSITY OF EDUCATION TẠP CHÍ KHOA HỌC JOURNAL OF SCIENCE KHOA HỌC TỰ NHIÊN VÀ CÔNG NGHỆ NATURAL SCIENCES AND TECHNOLOGY ISSN: 1859-3100 Tập 15, Số 3 (2018): 100-108 Vol. 15, No. 3 (2018): 100-108 Email: tapchikhoahoc@hcmue.edu.vn; Website: http://tckh.hcmue.edu.vn DEVELOPING AUTO-INDUCIBLE Pgrac57 PROMOTER IN BACILLUS SUBTILIS Phan Thi Phuong Trang1*, Tran Thi Anh Dao 2, Truong Thi Lan2, Nguyen Thuy My Linh2, Au Thi Hanh2, Dang Thanh Dung3, Nguyen Duc Hoang2 1 2 Laboratory of Molecular Biotechnology, University of Sciences, National University-HCMC Certer for Bioscience and Biotechnology, University of Sciences, National University-HCMC 3 Laboratory of Gene Technology and Applied Biotechnolgy, Faculty of Biotechnology, Ho Chi Minh Open University Received: 20/12/2017; Revised: 16/3/2018; Accepted: 26/3/2018 ABSTRACT Bacillus subtilis has many advantages such as: safe, non-pathogenic, endotoxinfree features. Therefore, B. subtilis has been widely ultilized in therapeutic proteins and important industrial enzymes production. Over the last few years, the potential application of B. subtilis for recombinant proteins synthesis has been significantly enhanced by using pHT - vector system with Pgrac promoter. In this study, we developed inducer-free expression vector for B. subitlis based on Pgrac57 promoter and used GFP protein as a reporter. The results showed that we successfully constructed inducer-free vector pHT1686 with Pgrac57 promoters. These vectors are totally able to express GFP protein without any inducer in B. subtilis strain 1012. Moreover, GFP expression level reached 11% of the total cellular proteins. Additionally, GFP activities are in the same range with the inducer-free vector. Keywords: Bacillus subtilis, GFP, Pgrac57, auto-inducible expression vector. TÓM TẮT Nghiên cứu sử dụng promoter Pgrac57 để tạo vectơ tự biểu hiện mang chỉ thị GFP cho Bacillus subtilis Bacillus subtilis có .