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Microalgal culture with digestate from methane fermentation concentration of digestate on growth of three microalgal species

In order to design a culture system for microalgal biomass production with a low cost and convenient cell collection, growth performance of mixtures of microalgal cells, including Euglena gracilis, Chlorella vulgaris, and Dunaliella tertiolecta cultured in a volume of 1 L were investigated at a PPFD of 300 µmol m-2 s-1 at the surface of the solution with continuous illumination at 30 °C. | Journal of Science and Technology 54 (5) (2016) 591-596 DOI: 10.15625/0866-708X/54/5/6752 MICROALGAL CULTURE WITH DIGESTATE FROM METHANE FERMENTATION - CONCENTRATION OF DIGESTATE ON GROWTH OF THREE MICROALGAL SPECIES Nguyen Tran Thien Khanh Faculty of Science, Technology and Environment, An Giang University, 18 Ung Van Khiem, Long Xuyen, An Giang province Email: nttkhanh@agu.edu.vn Received: 18 April 2015; Accepted for publication: 28 July 2016 ABSTRACT In order to design a culture system for microalgal biomass production with a low cost and convenient cell collection, growth performance of mixtures of microalgal cells, including Euglena gracilis, Chlorella vulgaris, and Dunaliella tertiolecta cultured in a volume of 1 L were investigated at a PPFD of 300 µmol m-2 s-1 at the surface of the solution with continuous illumination at 30 °C. Each culture container contained diluted digestate at concentrations of 5, 10, 15, 20, and 50 %. Sample cells for counting cell number were collected daily. Pseudospecific growth rates (µs) of each species at each depth were calculated as cellular multiplication rates using number of cells per time. The average µs of each species was highest in 5 % digestate. The average µs of all three microalgal species (0.035 h-1) was observed in all layers in 5 % digestate solution. The µs of each species was highest in 5 % digestate (0.048 h-1, 0.041 h-1, and 0.022 h-1, respectively for C. vulgaris, E. gracilis, and D. tertiolecta). In conclusion, E. gracilis, C. vulgaris, and D. tertiolecta showed the highest specific growth rate in 5 % digestate. Keywords: Chlorella, Dunaliella, digestate, Euglena, microalgae. 1. INTRODUCTION Microalgae have been used as sources of human foods, animal feeds, and pharmaceutical products, because of their marked ability to convert CO2 to biomass and their unique capacity to transform photosynthates to other useful compounds. Microalgae have also recently been used as a renewable biofuel source [1 – 3]. Euglena