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Rhodosporidium sp. growth in molasses medium and extraction of its astaxanthin by using HCL

The highest dried yeast biomass was 8.3682 g/l culture supernatant and astaxathin was 1.932 g/l culture supernatant by molasses medium containing 20 g/l sugar, 0.5 g/l ((NH4)2CO, 3 g/l MgSO4.7H2O and 10 % (v/v) inoculum. HCl extraction method was mixed 10 mg biomass: 1 ml HCl 0.6 N and incubated at 70 oC, 150 minutes. | Journal of Science and Technology 55 (1A) (2017) 8-18 DOI: 10.15625/2525-2518/55/1A/12377 Rhodosporidium sp. GROWTH IN MOLASSES MEDIUM AND EXTRACTION OF ITS ASTAXANTHIN BY USING HCl Quang-Vinh Tran1, 2, *, Quoc-Cuong Duong3, *, Dang-Khoa Tran3, Dai-Nghiep Ngo3 1 Institute of Tropical Biology, Thu Duc District, Ho Chi Minh City Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Street, Cau Giay District, Ha Noi 3 University of Science, VNU-HCM, 227 Nguyen Van Cu Street, District 5, Ho Chi Minh City 2 * Email: quangvinhgta@yahoo.com.vn; ndnghiep@hcmus.edu.vn Received: 30 October 2016; Accepted for publication: 30 May 2017 ABSTRACT Astaxanthin is classified as a xanthophyll-carotenoid, which is red-orange colour and powerful antioxidant activity. In this study, astaxanthin was collected from Rhodosporidum sp. by pilot culture (10 liters). Molasses medium was investigated with urea ((NH4)2CO), magnesium sulfate heptahydrate (MgSO4.7H2O) and potassium dihydrogen phosphate (KH2PO4) at different concentrations. Astaxanthin was extracted by using chlorhydric acid (HCl) method. The highest dried yeast biomass was 8.3682 g/l culture supernatant and astaxathin was 1.932 g/l culture supernatant by molasses medium containing 20 g/l sugar, 0.5 g/l ((NH 4)2CO, 3 g/l MgSO4.7H2O and 10 % (v/v) inoculum. HCl extraction method was mixed 10 mg biomass: 1 ml HCl 0.6 N and incubated at 70 oC, 150 minutes. Keywords: astaxanthin, Rhodosporidium sp., pilot, molasses medium, HCl extract. 1. INTRODUCTION Carotenoids, tetraterpenes, are organic pigments which play an important role in human health. Astaxanthin, 3,3’-dihydroxy- , carotene-4, 4’-dione, is a carotennoid, a red-orange pigment and a high antioxidant activity compound [1, 2]. It contains both the hydroxyl (-OH) and keto (C=O) groups on its ionone ring; therefore it is superior to most of the hydrophobic antioxidants and against UV-light effects, anti-cancer, prevents .