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Serological properties of Pseudomonas syringae pv phaseolicola isolates collected from Eskişehir

Thirty six bean seed samples collected from bean fields in Eskişehir-Türkiye were examined for detection and isolation of Pseudomonas syringae pv. phaseolicola. A polyclonal antiserum specific to P. s. pv. phaseolicola NCPPB 52 was raised in a rabbit and both biochemical and serological test were used for identification. | Tr. J. of Biology 22 (1998) 189-195 © TÜBİTAK Serological Properties of Pseudomonas syringae pv phaseolicola Isolates Collected From Eskişehir Dilek ERTUĞRUL, Kıymet GÜVEN Anadolu University, Faculty of Science, Department of Biology, Eskişehir-TURKEY Received: 06.06.1997 Abstract: Thirty six bean seed samples collected from bean fields in Eskişehir-Türkiye were examined for detection and isolation of Pseudomonas syringae pv. phaseolicola. A polyclonal antiserum specific to P. s. pv. phaseolicola NCPPB 52 was raised in a rabbit and both biochemical and serological test were used for identification. Heat-stable O antigens of 92 isolates identified as P. S. pv. phaseolicola together with authentic isolates of the bacterium were compared by Ouchterlony immunodiffusion test and all the isolates had a common antigenic structure. Key Words: Pseudomonas syringae pv. phaseolicola, halo blight, polyclonal antiserum, Ouchterlony immunodiffusion test. Eskişehir’den Toplanan Pseudomonas syringae pv. phaseolicola İzolatlarının Serolojik Özellikleri Özet: Eskişehir’deki fasulye ekim alanlarında toplanan 36 fasulye tohumunda Pseudomonas syringae pv. phaseolicola bakterisi aranmış ve izole edilmeye çalışılmıştır. P. s. pv. phaseolicola NCPPB 52 izolatı için spesifik poliklonal antiserum tavşanda üretilmiş ve identifikasyon için hem biyokimyasal hemde serolojik testler kullanılmıştır. Pseudomonas syringae pv. phaseolicola olarak teşhis edilen 92 izolat ile bu bakterinin standart suşlarının ısıya dayanıklı O antijenleri Ouchterlony immunodiffüzyon testi ile karşılaştırılmış ve bütün izolatların ortak antijenik yapıya sahip oldukları görülmüştür. Anahtar Sözcükler: Pseudomans syringae pv. phaseolicola, hale yanıklığı, poliklonal antiserum, Ouchterlony immunodiffusion testi Introduction Beans particularly dwarf beans (Phuseolus vulgaris ) is grown in nearly every part of Turkey and the consumption of beans was 2.7 kg per person in 1993 and it has been aimed to reach 3.5 kg in .