tailieunhanh - Role of precision motion control in brain cell research

Using an NSOM for confocal microscopy actin in the brain, nanopositioning, role of glial cells and actin in the brain,. As the main contents of the document "Role of precision motion control in brain cell research". Invite you to consult | Role of Precision Motion Control in Brain Cell Research by Katerina Moloni and Mike Szulczewski The close collaboration among a neuroscientist a laser microscopy instrumentation manufacturer and a precision nanopositioner manufacturer has resulted in an advanced near-field confocal optical microscope NCOM that is capable of imaging processes occurring within living cells in real NCOM combines the capabilities of confocal optical microscopy with near-field scanning optical microscopy NSOM . The result is the ability to image in real time processes that occur within a living nerve cell. Multiple technologies are merged in the NCOM including laser confocal photometry which makes high-speed 10KHz measurements of fluorescent signals over time. Near-field optical microscopy can attain optical resolution in the 20-50 nm range. However it has been difficult to achieve such resolution in living systems. Biological near-field microscopy has been used to image actin filaments in isolated nervous system glial cells with sub-50-nm resolution see sidebar . Under a grant from the NIH Gaithersburg MD Dr. Phil Haydon a neuroscientist at the University of Pennsylvania School of Medicine Philadelphia PA is using this technique on living cells to study calcium channels and single vesicles in isolated nerve terminals. Using an NSOM for confocal microscopy actin in the brain The NSOM is a scanning probe instrument that improves the resolution of visible-light microscopy from 500 nm to 20-50 nm. The NSOM principle is illustrated in Figure 1. An optical fiber probe tip illuminates the surface being examined. Except at the end the probe tip is coated with reflective aluminum and acts as an optical waveguide at the laser frequency. The uncoated end becomes a fine aperture 20-50 nm in diameter. When a sample is placed in close proximity the near-field to the aperture subdiffraction optical resolution can be achieved. Figure 2 is a photomicrograph taken using a conventional optical .

• Sinh học
• Role of precision motion control
• Brain cell research
• Using an NSOM
• Confocal microscopy actin in the brain
• Nanopositioning in the brain
• Role of glial cells and actin in the brain