tailieunhanh - Polymorphism in Exon 14 of antiviral resistant Mx Gene in Vietnamese indigenous chicken breeds

As Vietnam has rich resources of local chicken breeds, some of them are known having a certain level of infectious disease resistance. Knowledge of the single-nucleotide polymorphism in exon 14 of the chicken Mx gene will facilitate further researches on the resistant activity of Mx gene and its use for breeding of influenza virus resistant chicken varieties. This study aimed to investigate the exon 14 polymorphisms of the Mx gene in some representative indigenous chicken breeds collected from different locals in Vietnam. | J. Sci. Devel. Vol. 11 No. 5 635-640 Tạp chí Khoa học và Phát triển 2013 tập 11 số 5 635-640 POLYMORPHISM IN EXON 14 OF ANTIVIRAL RESISTANT MX GENE IN VIETNAMESE INDIGENOUS CHICKEN BREEDS Bùi Trần Anh Đào1 Đồng Thị Hồng Liên2 Nguyễn Thị Phương Thảo3 Daniel Desmecht4 Department of Veterinary Pathology Faculty of Veterinary Medicine 2Center for Experiment and Vocational Training Hanoi University of Agriculture Vietnam. 3Faculty of Biotechnology Hanoi University of Agriculture Vietnam 4Department of Morphopathology Faculty of Veterinary Medicine University of Liege Belgium Email btadao@ Received date Accepted date ABSTRACT The study was conducted to analyze the nucleotide polymorphisms of exon 14 Mx gene of seven Vietnamese indigenous chicken breedsL viz. Ac Dong Tao H mong Ho Mia Mong and Ri. The results revealed that the polymorphisms of exon 14 occured at two sites 2032th and 2159th . The non-synonymous substitution A G at nucleotide 2032th of Mx gene was found in all of seven indigenous breeds used in this study. In the same breed both of alleles A and G were present however allele A viral resistance was at a higher frequency than allele G viral susceptiblity . Particularly the allele A frequency of Ri and H mong was highest among 7 breeds examined and respectively . Comparative analysis of the deduced amino acids showed that 2032 A G polymorphism altered amino acid substitution at site 631 of Mx protein of Asparagine related to viral resistance by Serine lack of potent for viral resistance . Keywords Exon 14 indigenous chickens Mx gene nucleotide polymorphism Vietnam. Nghiên cứu tính đa hình Exon 14 gen MX ở một số giống gà bản địa của Việt Nam TÓM TẮT Nghiên cứu tính đa hình của exon 14 gen Mx được thực hiện trên 7 giống gà bản địa của Việt Nam bao gồm gà Ác Đông Tảo H Mông Hồ Mia Móng và Ri . Kết quả cho thấy tính đa hình của exon 14 xảy ra tại hai vị trí 2032 và 2159 . Sự thay thế A hoặc G tại vị trí .