tailieunhanh - Lecture Principles of biochemistry - Chapter 21 (part 2): Transcriptional regulation and RNA processing

Chapter 21 (part 2) - Transcriptional regulation and RNA processing. After completing this chapter, students will be able to: Know the functions of the beta, beta’ and alpha subunits; know the function of the sigma factor and how it affects the binding of the RNA polymerase complex to the promoter; know what concensus sequences are; know the names of the two major concensus sequences associated with the ER. Coli promoter region (-35 region and the Pribnow box (-10 region); | Chapter 21 (Part 2) Transcriptional Regulation and RNA Processing Regulation of Gene Expression AAAAAA 5’CAP mRNA RNA Processing RNA Degradation Protein Degradation Post-translational modification Active enzyme Activators and Repressors RNAP RNAP A co-A co-R +1 +1 R The Helix-Turn-Helix Motif contain two alpha helices separated by a loop with a beta turn The C-terminal helix fits in major groove of DNA; N-terminal helix stabilizes by hydrophobic interactions with C-terminal helix The Zn-Finger Motif Zn fingers form a folded beta strand and an alpha helix that fits into the DNA major groove. The Leucine Zipper Motif Forms amphipathic alpha helix and a coiled-coil dimer Leucine zipper proteins dimerize, either as homo- or hetero-dimers The basic region is the DNA-recognition site Basic region is often modeled as a pair of helices that can wrap around the major groove Transcription Regulation in Prokaryotes Binding of some trans-factors is regulated by allosteric modification lac operon Glucose is E. coli’s primary carbon source. But it can grow on different carbon sources. Diauxic growth of E. coli on a mixture of lactose + glucose. The lac I protein Operator and RNA Polymerase Bind at Overlapping Sites Inhibition of repression of lac operon by inducer binding to lacI Inducer : Allolactose, produced by side reaction of lacZ Lehninger: Principles of Biochemistry, 3rd Ed. IPTG is a Gratuitous Inducer Synthetic molecule Repression of the Tryptophan operon: A variation of the theme Catabolite Repression of lac Operon (Positive regulation) Molecular Cell Biology, 4th Edition, Lodish et. al. (2000) Why does the Lac Operon need an activator? Lac promoter has lousy promoter!!! tRNA Processing rRNA Processing Processing of Eukaryotic mRNA Splicing of Pre-mRNA Splicing of Pre-mRNA | Chapter 21 (Part 2) Transcriptional Regulation and RNA Processing Regulation of Gene Expression AAAAAA 5’CAP mRNA RNA Processing RNA Degradation Protein Degradation Post-translational modification Active enzyme Activators and Repressors RNAP RNAP A co-A co-R +1 +1 R The Helix-Turn-Helix Motif contain two alpha helices separated by a loop with a beta turn The C-terminal helix fits in major groove of DNA; N-terminal helix stabilizes by hydrophobic interactions with C-terminal helix The Zn-Finger Motif Zn fingers form a folded beta strand and an alpha helix that fits into the DNA major groove. The Leucine Zipper Motif Forms amphipathic alpha helix and a coiled-coil dimer Leucine zipper proteins dimerize, either as homo- or hetero-dimers The basic region is the DNA-recognition site Basic region is often modeled as a pair of helices that can wrap around the major groove Transcription Regulation in Prokaryotes Binding of some trans-factors is regulated by allosteric modification lac operon

• Sinh học
• Principles of biochemistry
• Lecture Principles of biochemistry
• Nguyên lý hóa sinh
• Biochemical compounds
• Transcriptional regulation
• RNA processing
• Ebook Principles of biochemistry
• Principles of biochemistry (4th edition)
• The glyoxylate cycle
• Fatty acid catabolism
• Electron Transfer reactions
• Evolutionary foundations
• Protein interactions modulated
• Nucleic acid structure
• Biological systems
• Biological macromolecules
• Chemical principles
• Properties of water
• Hydrogen bonding
• Amino acids
• Amino acid structure
• Protein purification
• Cell extract
• Protein classification
• Protein 3 Dimensional structure
• Protein folding
• Protein 3 D structure
• 3 D structure
• Oxygen binding curve
• Enzyme kinetics
• Rate constants
• Catalytic Power
• Classes of enzymes
• Inhibitor kinetics
• Enzyme regulation
• Allosteric regulation
• Enzyme action
• Mechanisms of enzyme action
• Ions Cofactors
• Metal ion
• Biotin cofactor
• Conformation of monosaccharides
• Carbohydrate classifications
• Oligo carbohydrate
• Polysaccharides carbohydrate
• Lipid subclasses
• Acyl lipids
• Fluid mosaic model
• membrane transporters
• Metabolism proceed
• Anaeorbic process
• Triose Phosphate Isomerase
• Regulation of glycolysis
• Control points in glycolysis
• Citric acid cycle
• TCA cycle