tailieunhanh - Bacterial strains and culture conditions

Bacterial strains and culture conditions: Grape pomace is a natural product rich in dietary fiber and polyphenols. A major part of dietary polyphenols is not absorbed in the small intestine and can interact with colonic microbiota. The influence of grape polyphenols on Lactobacillus acidophilus CECT 903 growth was investigated through agar diffusion assays and cultures in liquid media. Grape phenolic extracts and some standards of phenolic compounds (caffeic acid, gallic acid, tannic acid, catechin, epicatechin, and quercetin) were assayed. All phenolic compounds tested did not exert an inhibitory effect on L. acidophilus growth at a maximum concentration of 5000 μg/ disk in agar diffusion assays. | International Journal of Food Microbiology 136 2009 119-122 Contents lists available at ScienceDirect International Journal of Food Microbiology journal homepage locate ijfoodmicro Short communication Stimulatory role of grape pomace polyphenols on Lactobacillus acidophilus growth Deisy Hervert-Hernandez a Concepcion Pintado b Rafael Rotger b Isabel Gonia a Nutrition and Gastrointestinal Health Unit Department of Nutrition and Bromatology I Faculty of Pharmacy Universidad Complutense de Madrid 28040 Madrid Spain b Department of Microbiology II Faculty of Pharmacy Universidad Complutense de Madrid 28040 Madrid Spain ARTICLE INFO ABSTRACT Article history Received 27 April 2009 Received in revised form 2 September 2009 Accepted 18 September 2009 Keywords Polyphenols Lactobacillus acidophilus Grape pomace Grape pomace is a natural product rich in dietary fiber and polyphenols. A major part of dietary polyphenols is not absorbed in the small intestine and can interact with colonic microbiota. The influence of grape polyphenols on Lactobacillus acidophilus CECT 903 growth was investigated through agar diffusion assays and cultures in liquid media. Grape phenolic extracts and some standards of phenolic compounds caffeic acid gallic acid tannic acid catechin epicatechin and quercetin were assayed. All phenolic compounds tested did not exert an inhibitory effect on L. acidophilus growth at a maximum concentration of 5000 pg disk in agar diffusion assays. In addition a stimulatory trend in bacterial growth was observed in both grape phenolic extracts and tannic acid. The major finding was that grape pomace phenolic extract 1 mg mL induced a significant biomass increase of L. acidophilus grown in liquid culture media. Further research into the interaction between phenolic compounds and other intestinal bacteria as well as healthy consequences is required. 2009 Elsevier . All rights reserved. 1. Introduction Grape pomace is a natural product rich in dietary