tailieunhanh - Study of dracaena angustifolia - III: New steroidal saponins from roots and rhizomes, and their antiproliferative activity

The MeOH extract of Nam ginseng (roots and rhizomes of Dracaena angustifolia) afforded two new furostanol saponins, named namonins E and F (1 and 2), along with another four known steroidal saponins (3 - 6). Their structures were determined on basis of spectral analyses and chemical methods. All compounds were tested for their antiproliferative activity against murine colon 26-L5 carcinoma, human HT-1080 fibrosar- coma and B-16 BL6 melanoma cells. Compounds 10, 11 and 14 showed potent antiprolife- rative activity against HT-1080 fibrosarcoma cells, having IC50 values of µM, µM and µM, respectively, comparable to that of doxorubicin. | Journal of Chemistry Vol. 42 3 P. 371 - 375 2004 STUDY OF DRACAENA ANGUSTIFOLIA III - NEW STEROIDAL SAPONINS FROM ROOTS AND RHIZOMES AND THEIR ANTIPROLIFERATIVE ACTIVITY Received 14-5-2003 TRAN LE QUAN1 TRAN KIM QUI1 SHIGETOSHI KADOTA2 1College of Natural Sciences National University Ho Chi Minh City Ho Chi Minh City institute of Natural Medicine Toyama Medical Pharmaceutical University Toyama Japan SUMMARY The MeOH extract of Nam ginseng roots and rhizomes of Dracaena angustifolia afforded two new furostanol saponins named namonins E and F 1 and 2 along with another four known steroidal saponins 3 - 6 . Their structures were determined on basis of spectral analyses and chemical methods. All compounds were tested for their antiproliferative activity against murine colon 26-L5 carcinoma human HT-1080 fibrosarcoma and B-16 BL6 melanoma cells. Compounds 10 11 and 14 showed potent antiproliferative activity against HT-1080fibrosarcoma cells having IC50 values of uM uM and ụM respectively comparable to that of doxorubicin. I - INTRODUCTION Dracaena angustifolia Roxb. Dracaena-ceae is locally known as Nam ginseng ginseng from the South in Quang Nam province. Its underground parts are used as tonic and for treatment of leukemia 1 . In our coninuing studies on Vietnamese medicinal plants we have examined the constituents of Nam ginseng D. angustifolia and reported an isolation of three new spirostanol sapogenins and four new spiros-tanol saponins 2 3 . Further investiga-tion led to isolation of additional two new steroidal saponins named namonins E and F 1 and 2 along with another four known 3 - 6 . This paper reports the isolation and structure elucidation of these new compounds together with antiproliferative activity of all compounds isolated. II - EXPERIMENT General Experimental Procedures and Plant Material were the same as those reported in our previous paper 2 3 . Extraction and Isolation. The MeOH fraction was chromatographed on silica gel with .