tailieunhanh - Báo cáo Y học: Barrier passage and protein dynamics in enzymatically catalyzed reactions

This review describes studies of particular enzymatically catalyzed reactions to investigate the possibility that catalysis is mediated by protein dynamics. That is, evolution has crafted the protein backbone of the enzyme to direct vibrations in such a fashion to speed reaction. The review presents the theoretical approach we have used to investigate this problem, but it is designed for the nonspecialist. | Eur. J. Biochem. 269 3103-3112 2002 FEBS 2002 doi MINIREVIEW Barrier passage and protein dynamics in enzymatically catalyzed reactions Dimitri Antoniou1 Stavros Caratzoulas1 I C. Kalyanaraman1 Joshua S. Mincer1 and Steven D. Schwartz1 2 1 Department of Biophysics Albert Einstein College of Medicine Bronx NY USA 2Department of Biochemistry Albert Einstein College of Medicine Bronx NY USA This review describes studies of particular enzymatically catalyzed reactions to investigate the possibility that catalysis is mediated by protein dynamics. That is evolution has crafted the protein backbone of the enzyme to direct vibrations in such a fashion to speed reaction. The review presents the theoretical approach we have used to investigate this problem but it is designed for the nonspecialist. The results show that in alcohol dehydrogenase dynamic protein motion is in fact strongly coupled to chemical reaction in such a way as to promote catalysis. This result is in concert with both experimental data and interpretations for this and other enzyme systems studied in the laboratories of the two other investigators who have published reviews in this issue. Keywords protein dynamics enzyme catalysis tunneling promoting vibration promoting mode. INTRODUCTION The transmission of an atom or group of atoms from the reactant region of a reaction to the product region under the control of an enzyme is central to biochemistry. The manner in which the enzyme speeds this transfer is in some cases still not clear. What is known is the end effect enzymatic reactions occur at rates many orders of magnitude more rapid than the correspondingsolution phase reactions. This review will describe work recently completed in our group that has focused on examiningthe possibility that protein dynamics may in some enzymes play a central role in helping to produce the catalytic effect. These types of motions which we have termed rate promoting vìt ríitíons are .

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