tailieunhanh - Báo cáo Y học: Expression of the aspartate/glutamate mitochondrial carriers aralar1 and citrin during development and in adult rat tissues

Aralar1 and citrin are members of the subfamily of calciumbinding mitochondrial carriers and correspond to two isoforms of the mitochondrial aspartate/glutamate carrier (AGC). These proteins are activated by Ca2+ acting on the external side of the inner mitochondrial membrane. Although it is known that aralar1 is expressed mainly in skeletal muscle, heart and brain, whereas citrin is present in liver, kidney and heart, the precise tissue distribution of the two proteins in embryonic and adult tissues is largely unknown | Eur. J. Biochem. 269 3313-3320 2002 FEBS 2002 doi Expression of the aspartate glutamate mitochondrial carriers aralarl and citrin during development and in adult rat tissues Araceli del Arco1 3 Julian Morcillo2 Juan Ramon Martinez-Morales2 Carmen Galian1 Vera Martos1 Paola Bovolenta2 and Jorgina Satrustegui1 1Departamento de Biologia Molecular Centro de Biologia Molecular Severo Ochoa Universidad Autonoma de Madrid Spain 2Departamento de Neurobiologia del Desarrollo Instituto Cajal Consejo Superior de Investigaciones Cientficas Madrid Spain 3Facultad de Ciencias del Medio Ambiente Universidad de Castilla La Mancha Toledo Spain Aralar1 and citrin are members of the subfamily of calcium-binding mitochondrial carriers and correspond to two isoforms of the mitochondrial aspartate glutamate carrier AGC . These proteins are activated by Ca2 acting on the external side of the inner mitochondrial membrane. Although it is known that aralar1 is expressed mainly in skeletal muscle heart and brain whereas citrin is present in liver kidney and heart the precise tissue distribution of the two proteins in embryonic and adult tissues is largely unknown. We investigated the pattern of expression of aralar1 and citrin in murine embryonic and adult tissues at the mRNA and protein levels. hl situ hybridization analysis indicates that both isoforms are expressed strongly in the branchial arches dermomyotome limb and tail buds at early embryonic stages. However citrin was more abundant in the ectodermal components of these structures whereas aralarl had a predominantly mesenchymal localization. The strong expression of citrin in the liver was acquired postnatally whereas the characteristic expression of aralar1 in skeletal muscle was detected at E18 and that in the heart began early in development E11 and was preferentially localized to auricular myocardium in late embryonic stages. Aralar1 was also expressed in bone marrow T-lymphocytes and macrophages

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