tailieunhanh - Báo cáo Y học: Expression pattern in the antennae of a newly isolated lepidopteran Gq protein a subunit cDNA

From the antennae of the moth Mamestra brassicae, we have identified a lepidopteran G protein a subunit belonging to the Gq family, through immunological detection in crude antennal extract and antennal primary cell cultures, followed by molecular cloning. The complete cDNA sequence (1540 bp) contains an open reading frame encoding a protein of 353 amino acids. This deduced sequence possesses all of the characteristics of the Gq family and shares a very high degree of amino-acid sequence identity with vertebrate (80% with mouse or human Gqa) and invertebrate subunits (varying between 60 and 87% for Gqa from organisms as diverse. | Eur. J. Biochem. 269 2133-2142 2002 FEBS 2002 doi Expression pattern in the antennae of a newly isolated lepidopteran Gq protein a subunit cDNA Emmanuelle Jacquin-Joly1 Marie-Christine Francois1 Michael Burnet2 Philippe Lucas1 Franck Bourrat3 and Rosario Maida4 1INRA Unite de Phytopharmacie et Mediateurs Chimiques Route de Saint-Cyr Versailles cedex France 2Sympore GmbH Reutlingen Germany 3UPR 2197 DEPSN Institut de Neurosciences A. Fessard CNRS Gif-sur-Yvette France 4Max-Planck-Institut fur Verhaltensphysiologie Seewiesen Germany From the antennae of the moth Mamestra brassicae we have identified a lepidopteran G protein a subunit belonging to the Gq family through immunological detection in crude antennal extract and antennal primary cell cultures followed by molecular cloning. The complee cDNA sequence 1540 bp contains an open reading frame encoding a protein of 353 amino acids. This deduced ses uence possesses all of the characteristics of the Gq family and shares a very high degree of amino-acid sequence identity with vertebrate 80 with mouse or human Gqa and invertebrate subunits varying between 60 and 87 for Gqa from organisms as diverse as sponge and Drosophila . The ex pression pattern of the Gq subunit in adult antennae was associated with the olfactory sensilla suggesting a specific role in olfaction. These data provide molecular evidence for a component of the phosphoinositide signaling pathway in moth antennae this G protein a subunit may be involved in the olfaction transduction process through interaction with G-protein-coupled rerbptors stimulating the phospholipase C mediated second messenger pathway. Keywords G protein a subunit olfaction Lepidoptera in situ hybridization. For insects olfaction plays an essential role in processing chemical signals from the environment leading to the detection of food reproductive partners oviposition sites hosts prey or predators. n particular pheoomone perception in moths has .

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