tailieunhanh - Báo cáo khoa học: "Controlled Authoring of Biological Experiment Reports"

Each protein has a specific behavior and many parameters can vary (Stevens 2000). Protein'eXpert 1 has developed an expertise to determine optimal production conditions for recombinant proteins, and it provides several products and services in this field. One of these services is called the feasibility study which is a complete and standardized protein production study including cloning, expression and solubility tests, cell fractionation, purification, refolding assay (when necessary), quality control and delivery of 1-10 mg of soluble proteins. . | Controlled Authoring of Biological Experiment Reports Caroline Brun Xerox Research entre Europe Eric Fanchon Institut de Biologic Structurale Abstract We give a demonstration of an application of XRCE s controlled text authoring system MDA to biological experiment reports. This work is the result of a collaboration between XRCE s Document Content Models team CNRS s Institut de Biologie Structurale and Protein e Xpert a company specialized in biotechnology based in Grenoble. We start with a brief presentation of the partners involved and their respective goals. We then give some technical background on the MDA system. Some novel features of the application are discussed in particular how MDA can be used for integrating the formalization of an experimental protocol with its associated textual documentation. 1 Partners involved Protein eXpert Whereas the human genome sequencing has now been completed the formidable task remains of understanding the function of proteins encoded by genes. For this reason the production of recombinant proteins has become an essential aspect of biomedical and biotechnology research that is exploratory therapeutic research functional and structural studies. Genes are coding sequences of DNA molecules and are the templates from which proteins are synthesized. Proteins are long linear molecules which fold into a well-defined 3-dimensional structure. The structure of a protein determines its biological function. The synthesis of proteins from genes is performed by the complex molecular machinery present in living cells. Recombinant DNA technology is a set of procedures that allow the production of a protein from a given organism by another organism which can be easily manipulated and cultured. In appropriate conditions these host cells arc forced to synthesize the protein that has been artificially incorporated. Thus by Marc Dy metman Xerox Research Centre Europe .