tailieunhanh - Báo cáo Y học: Ductus ejaculatorius peptide 99B (DUP99B), a novel Drosophila melanogaster sex-peptide pheromone

We have characterized a glycosylated, 31 amino-acid peptide of 4932 Da isolated from Drosophila melanogaster males. The mature peptide contains a sugar moiety of 1184 Da at a NDT consensus glycosylation site and a disulfide bond. It is synthesized in the male ejaculatory duct via a 54 amino-acid precursor containing an N-terminal signal peptide and Arg-Lys at the C-terminus which is cleaved off during maturation. The gene contains an intron of 53 bp and is localized in the cytological region 99B of the D. melanogaster genome | Eur. J. Biochem. 269 989-997 2002 FEBS 2002 Ductus ejaculatorius peptide 99B DUP99B a novel Drosophila melanogaster sex-peptide pheromone Philiooe Saudan1. Klaus Hauck1 Matthias Sỏller1 Yves Choffat1 Michael Ottiaer1 Michael Soỏrri1 Zhaobing Ding1 Daniel Hess2 t Peter M. Gehrig2 Stefan Klauser2 Peter Hunziker2 and Eric Kubli1 1Zoologisches Institut Universitdt Zurich-Irchel Zurich Switzerland 2Biochemisches Institut der Universitat Zurich-Irchel Zurich Switzerland We have characterized a glycosylated 31 amino-acid peptide of 4932 Da isolated from Drosophila melanogaster males. The mature peptide contains a sugar moiety of 1184 Da at a NDT consensus glycosylation site and a disulfide bond. It is synthesized in the male ejaculatory duct via a 54 amino-acid precursor containing an N-terminal signal peptide and Arg-Lys at the C-terminus which is cleaved off during maturation. The gene contains an intron of 53 bp and is localized in the cytological region 99B of the D. melanogaster genome. The peptide is therefore named DUP99B for ductus ejaculatorius peptide cytological localization 99B . The C-terminal parts of mature DUP99B and D. melanogaster sex-peptide ACP70A are highly homologous. Injected into virgin females DUP99B elicits the same postmating responses as sex-peptide increased oviposition reduced receptivity . These effects are also induced by de-glycosylated native peptide or synthetic DUP99B lacking the sugar moiety. Presence of the glycosyl group however decreases the amount needed to elicit the postmating responses. Homologies in the coding regions of the two exons of DUP99B and sex-peptide respectively suggest that the two genes have evolved by gene duplication. Thus we consider these two genes to be members of the new sex-peptide gene family. Keywords Drosophila melanogaster ductus ejaculatorius oviposition receptivity sex-peptides. In many insects the reproductive behaviour of females is influenced by peptides and other substances synthesized in the male