tailieunhanh - Ebook Molecular histopathology and tissue biomarkers in drug and diagnostic development: Part 2

(BQ) Part 1 book "Molecular histopathology and tissue biomarkers in drug and diagnostic development" presentation of content: Final recommendations, three companion diagnostic development paths, implementing a multi analyte immunohistochemistry panel into a drug development program, cutpoint methods in digital pathology and companion diagnostics,. | Methods in Pharmacology and Toxicology (2015): 141–152 DOI © Springer Science+Business Media New York 2014 Published online: 25 October 2014 Development of a Tissue Image Analysis Algorithm for Celiac Drug Development Erik Hagendorn, Christa Whitney-Miller, Aaron Huber, and Steven J. Potts Abstract Celiac disease, an immune-mediated condition related to gluten sensitivity, is gaining pharmaceutical development interest. Recent conversations with the US Food and Drug Administration (FDA) indicate pathology readouts from intestinal biopsies will continue to be a primary clinical trial endpoint. The existing methodology, the Marsh-Oberhuber score, is a qualitative assessment of celiac severity, combining a morphological criterion known as villous height to crypt depth ratio (VC), with an assessment of localized immune response, manually estimating intraepithelial lymphocyte (IEL) counts. A stereology and image analysis based whole slide imaging methodology was developed for use in CLIA-based clinical trials. Experimental Design: A series of ten normal and ten abnormal patient small bowel biopsies were manually evaluated by two pathologists to determine celiac disease (CD) state using the standard Marsh score. Two quantitative methods were developed—an automated stereological methodology was used to evaluate surface area on whole slide images and an image analysis complementary approach. Methods: Stereology line probes were used to count one-dimensional “hits” on points at the distal ends of the lines which exist over reference tissue area, and “cuts” through the two-dimensional range of the line as it passes through the epithelium of the reference tissue to background, or vice versa. Results: There was strong concordance between the pathologist scores, and the automated stereology analysis, with the automated approaches able to sufficiently delineate intermediate grades of disease, normally more difficult in visual assessments. Conclusion: The .

• Y khoa - Dược
• Molecular histopathology
• Tissue biomarkers in drug
• Diagnostic development
• Final recommendations
• Three companion diagnostic development paths
• Companion diagnostics
• Materials and methods
• Heterogeneity approaches
• Image analysis tools
• Histopathology in mouse models
• Quantification of blood
• Histopathology of cancer
• Childhood cancers
• Molecular biology
• Alkylating agents
• Preclinical pharmacology
• lead drugs
• BMC Cancer
• Hodgkin lymphoma
• Diffuse large B cell lymphoma
• Particular molecular features
• B cell markers
• EGFR mutation testing
• Molecular diagnostics
• Non small cell lung cancer
• Analytical validation
• Microsatellite instability
• Lynch syndrome
• Genetic medicine