tailieunhanh - UNDERSTANDING AND MANAGING CELL CULTURE CONTAMINATION

In setting up a national statistical organization, management must come to grips with a number of key issues concerning dissemination to the media. It might be preferable to develop policies on many of these issues, which include: Allocating resources: The national statistical organization must first decide how much it wants to spend on media relations. Financial resources are not infinite. An organization’s wish to be as helpful to the media as possible must be counterbalanced by a program that fits within its available budget. . | Understanding and Managing Cell Culture Contamination Technical Bulletin John Ryan . Corning Incorporated Life Sciences 900 Chelmsford St. Lowell MA 01851 Table of Contents What Are the Major Cell Culture Contaminants .2 What Are the Sources of Biological Contaminants . 8 How Can Cell Culture Contamination Be Controlled .10 A Final References .21 Cell Culture Protocols and Technical Introduction No cell culture problem is as universal as that of culture loss due to contamination. All cell culture laboratories and cell culture workers have experienced it. Culture contaminants may be biological or chemical seen or unseen destructive or seemingly benign but in all cases they adversely affect both the use of your cell cultures and the quality of your research. Contamination problems can be divided into three classes Minor annoyances when up to several plates or flasks are occasionally lost to contamination Serious problems when contamination frequency increases or entire experiments or cell cultures are lost Major catastrophes contaminants are discovered that call into doubt the validity of your past or current work. The most obvious consequence of cell culture contamination is the loss of your time money for cells culture vessels media and sera and effort spent developing cultures and setting up experiments. However the less obvious consequences are often more serious Table 1 . First there are the adverse effects on cultures suffering from undetected chemical or biological contaminants. These hidden cryptic contaminants can achieve high densities altering the growth and characteristics of the cultures. Worse yet are the potentially inaccurate or erroneous results obtained by unknowingly working with these cryptically contaminated cultures. CORNING Products such as vaccines drugs or monoclonal antibodies manufactured by these cultures will probably be useless. For some researchers the most serious .

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