tailieunhanh - Báo cáo Y học: Novel fish hypothalamic neuropeptide Cloning of a cDNA encoding the precursor polypeptide and identification and localization of the mature peptide

Recently, we identified novel avian and amphibian hypothalamic neuropeptides that inhibited gonadotropin release and stimulated growth hormone release. They were characterized by a similar structure including theC-terminal LPLRF-NH2motif. To clarify that the expression of these novel hypothalamicneuropeptides is aconservedproperty in vertebrates, we characterized a cDNA encoding a similar novel peptide, having LPLRF-NH2 from the goldfish brain, by a combination of 3¢and 5¢rapid amplification of cDNA ends (RACE). . | Eur. J. Biochem. 269 6000-6008 2002 FEBS 2002 doi Novel fish hypothalamic neuropeptide Cloning of a cDNA encoding the precursor polypeptide and identification and localization of the mature peptide Kaori Sawada1 2 Kazuyoshi Ukena1 2 Honoo Satake3 Eiko Iwakoshi3 Hiroyuki Minakata3 and Kazuyoshi Tsutsui1 2 1 Laboratory of Brain Science Faculty of Integrated Arts and Sciences Hiroshima University Higashi-Hiroshima Japan 2Core Research of Evolutional Science and Technology CREST Japan Science and Technology Corporation Tokyo Japan and 3Suntory Institute for Bioorganic Research Wakayamadai 1-1-1 Shimamoto-cho Mishima-gun Osaka Japan Recently we identified novel avian and amphibian hypothalamic neuropeptides that inhibited gonadotropin release and stimulated growth hormone release. They were characterized by a similar structure including the C-terminal LPLRF-NH2 motif. To clarify that the expression of these novel hypothalamic neuropeptides is a conserved property in vertebrates we characterized a cDNA encoding a similar novel peptide having LPLRF-NH2 from the goldfish brain by a combination of 3 and 5 rapid amplification of cDNA ends RACE . The deduced peptide precursor consisted of 197 amino acid residues encoding three putative peptide sequences that included -LPXRF where X is L or Q at their C-termini. Mass spectrometric analyses revealed that a tridecapeptide SGTGLSATLPQRF-NH2 was derived from the precursor in the brain as an endogenous ligand. Southern blotting analysis of reverse-transcriptase-mediated PCR products demonstrated a specific expression of the goldfish peptide gene in the diencephalon. In situ hybridization revealed the cellular localization of goldfish peptide mRNA in the nucleus posterioris periventricularis in the hypothalamus. Immunoreactive cell bodies were also restricted to the the nucleus posterioris periventricularis and the nervus ter-minalis and immunoreactive fibers were distributed in several brain regions

TỪ KHÓA LIÊN QUAN
crossorigin="anonymous">
Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.