tailieunhanh - Báo cáo Y học: Antibacterial and antifungal properties of a-helical, cationic peptides in the venom of scorpions from southern Africa

Two novel pore-forming peptides have been isolated from the venom of the South-African scorpion Opistophtalmus carinatus. These peptides,designated opistoporin 1 and 2, differ by only one amino acid and belong to a group of a-helical,cationic peptides. For the first time,a comparison of the primary structures ofa-helical pore-forming peptides from scorpion venom was undertaken. | Eur. J. Biochem. 269 4799- 810 2002 FEBS 2002 doi Antibacterial and antifungal properties of a-helical cationic peptides in the venom of scorpions from southern Africa Leentje Moerman1. Suzanne Bosteels1 Wim Noone1 Jean Willems1 Elke Clvnen2. Liliane Schoofs2 Karin Thevissen3 Jan Tytgat4 Johan Van Eldere5 Jurg van der Walt6 and Fons Verdonck1 1 Interdisciplinary Research Center Katholieke Universiteit Leuven Campus Kortrijk Kortrijk laboratory for Developmental Physiology and Molecular Biology Katholieke Universiteit Leuven Leuven . Janssens Laboratory of Genetics Katholieke Universiteit Leuven Heverlee 4Laboratory of Toxicology Katholieke Universiteit Leuven Leuven 5Laboratory for experimental Microbiology Rega Institute Katholieke Universiteit Leuven Leuven Belgium 6Department of Physiology University of Potchefstroom Potchefstroom South Africa Two novel pore-forming peptides have been isolated from the venom of the South-African scorpion Opistophtalmus carinatus. These peptides designated opittoporin 1 and 2. differ by only one amino acid and belong to a group of a-helical caSýomc pepidles. For the ÍĨstt iime. a oomaasison of the primary structures of a-helical pore-forming peptides from scorpion venom was undertaken. This analysis revealed that peptides in the range of 40-50 amino acids contain a typical scorpion conserved sequence S x 3KxWxS x 5L. An extensive study of biological activity of synthesized opistoporin 1 and parabutoporin a poreforming peptide previously isolated from the venom of the South-African scorpion Parabuthus schlechteri was undrr-taken to investigate an eventual cell-selective effect of the peptides. Opistoporin 1 and parabutoporin were most active in inhibiting growth of Gram-negative bacteria im while melittin and mastoparan two well-nnoywi ctpolyiic peptides were more effecii ve again st Gram-posí ii ve bac teri a in the same concentration range. In addition the iteitie tes showed .

crossorigin="anonymous">
Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.