tailieunhanh - Báo cáo Y học: Structural determination of lipid A of the lipopolysaccharide from Pseudomonas reactans A pathogen of cultivated mushrooms

The chemical structure of lipid A from the lipopolysaccha-ride of the mushroom-associated bacterium Pseudomonas reactans,a pathogen of cultivated mushroom, was elucida-ted by compositional analysis and spectroscopic methods (MALDI-TOF and two-dimensional NMR). The sugar backbone was composed of theb-(1¢fi6)-linkedD-gluco-samine disaccharide 1-phosphate. | Eur. J. Biochem. 269 2498-2505 2002 FEBS 2002 doi Structural determination of lipid A of the lipopolysaccharide from Pseudomonas reactans A pathogen of cultivated mushrooms Alba Silipo1 Rosa Lanzetta1 Domenico Garozzo2 Pietro Lo Cantore3 Nicola Sante lacobellis3 Antonio Molinaro1 Michelangelo Parrilli1 and Antonio Evidente4 1 Dipartimento di Chimica Organica e Biochimica Universita degli Studi di Napoli Federico II Napoli Italy 2Istituto per la Chimica e la Tecnologia dei Materiali Polimerici Catania Italy 3Dipartimento di Biologia Difesa e Biotecnologie Agro Forestali Unirersila degli Studi della Basilicata Potenza Italy 4Dipartimento di Scienze Chimico-Agrarie Universita di Napoli Federico II Napoli Italy The chemical structure of lipid A from the lipopolysaccharide of the mushroom-associated bacterium Pseudomonas reactans a pathogen of cultivated mushroom was elucidated by compositional analysis and spectroscopic methods MALDI-TOF and two-dimensional NMR . The sugar backbone was composed of the b- 1 fi 6 -linked D-gluco-samine disaccharide 1-phosphate. The lipid A fraction showed remarkable heterogeneity with respect to the fatty acid and phosphate composition. The major species are hexacylated and pentacylated lipid A bearing the R -3-hydroxydodecanoic acid C12 0 3OH in amide linkage and a R -3-hydroxydecanoic C10 0 3OH in ester linkage while the secondary fatty acids are present as C12 0 and or C12 0 2-OH . A nonstoichiometric phosphate substitution at position C-4 of the distal 2-deoxy-2-amino-glucose was detected. Interestingly the pentacyl lipid A is lacking a primary fatty acid namely the C10 0 3-OH at position C-3 . The potential biological meaning of this peculiar lipid A is also discussed. Keywords cultivated mushrooms lipid A MALDI-TOF NMR Pseudomonas reactans. Lipopolysaccharides LPS of Gram-negative bacteria are composed of three genetically and structurally distinct regions the O-specific polysaccharide O-chain .

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