tailieunhanh - Báo cáo Y học: Structure of the O-polysaccharide and classification of Proteus mirabilis strain G1 in Proteus serogroup O3

TheO-chainpolysaccharide of the lipopolysaccharide (LPS) of a previously nonclassi®ed strain ofProteus mirabilis termed G1 was studied by sugar analysis and 1 Hand13 C NMR spectroscopy, including 2D COSY, TOCSY, rota-ting-frameNOE(ROESY),H-detected 1 H, 13 CHMQC,and heteronuclear multiple-bond correlation (HMBC) experi-ments. | Eur. J. Biochem. 269 1406-1412 2002 FEBS 2002 Structure of the O-polysaccharide and classification of Proteusmirabilis strain G1 in Proteus serogroup 03 Zygmunt Sidorczyk1 Krystyna Zych1 Filip V. Toukach2 Nikolay P. Arbatsky2 Agnieszka Zablotni1 Alexander S. Shashkov2 and Yuriy A. Knirel2 1 Department of General Microbiology Institute of Microbiology and Immunology University of Lodz Poland . Zelinsky Institute of Organic Chemistry Russian Academy of Sciences Moscow Russian Federation The O-chain polysaccharide of the lipopolysaccharide LPS of a previously nonclassified strain of Proteus mirabilis termed G1 was studied by sugar analysis and H and 13C NMR spectroscopy including 2D COSY TOCSY rotating-frame NOE ROESY H-detected 1H 13CHMQC and heteronuclear multiple-bond correlation HMBC experiments. The following structure of the polysaccharide was established a-D-GalpA6 L-Lys 1 i 4 3 -P-D-GalpNAc- l 6 -P-D-GalpNAc- l 4 -P-D-GlcpA- l where D-GalA6 L-Lys stands for N - D-galacturonoyl -L-lysine. The structure of the O-polysaccharide of P. mirabilis G1 is similar but not identical to that of P. mirabilis S1959 and OXK belonging to serogroup O3. Immunochemical studies with P. mirabilis G1 and S1959 anti- O-polysaccharide sera revealed close LPS-based serological relatedness of P. mirabilis G1 and S1959 and therefore it was suggested to classify P. mirabilis G1 in serogroup O3 as a subgroup. P. mirabilis G1 and S1959 anti- O-polysaccharide sera also cross-reacted with LPS of P. mirabilis strains from two other serogroups containing D-GalA6 L-Lys in the O-polysaccharide or in the core region. Keywords Proteus mirabilis O-polysaccharide lipopolysaccharide N - D-galacturonoyl -L-lysine serogroup. Much has been written about the taxonomy of Proteus since the original publication by Hauser in 1885 who established the genus 1 . Currently the genus Proteus consists of hve named species P. mirabilis P. penneri P. vulgaris P. myxofaciens and P. hauseri and three unnamed .

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