tailieunhanh - Báo cáo Y học: Evaluation of two biosynthetic pathways to d-aminolevulinic acid in Euglena gracilis

d-Aminolevulinic acid (ALA), which is an intermediate in the biosynthesis of chlorophyll a, can be biosynthesized via theC5pathway and the Sheminpathway inEuglena gracilis. Analysis of the 13 C-NMR spectrum of 13 C-labeled methyl pheophorbidea, derived from 13 C-labeled chlorophylla biosynthesized fromD-[1-13 C]glucose byE. gracilis,provid-ed evidence suggesting that ALA incorporated in the 13 C-labeled chlorophyllawas synthesized via both the C5 pathway and the Shemin pathway in a ratio of between and to one | Eur. J. Biochem. 269 291-297 2002 FEBS 2002 Evaluation of two biosynthetic pathways to d-aminolevulinic acid in Euglena gracilis Katsumi lida Ippei Mimura and Masahiro Kajiwara Department of Medicinal Chemistry Meiji Pharmaceutical University Kiyose-shi Tokyo Japan S-Aminolevulinic acid ALA which is an intermediate in the biosynthesis of chlorophyll a can be biosynthesized via the C5 pathway and the Shemin pathway in Euglena gracilis. Analysis of the 13C-NMR spectrum of 13C-labeled methyl pheophorbide a derived from 13C-labeled chlorophyll a biosynthesized from D- 1-13C glucose by E. gracilis provided evidence suggesting that ALA incorporated in the 13C-labeled chlorophyll a was synthesized via both the C5 pathway and the Shemin pathway in a ratio of between and to one. The methoxyl carbon of the methoxycarbonyl group at C-132 of chlorophyll a was labeled with 13C. The phytyl moiety of chlorophyll a was labeled on C-P2 C-P31 C-P4 C-P6 C-P71 C-P8 C-P10 C-P111 C-P12 C-P14 C-P151 andC-P16. Keywords S-aminolevulinic acid C5 pathway Shemin pathway Euglena gracilis 13C-NMR. ỗ-Aminolevulinic acid ALA Fig. 1 3 which is an intermediate in the biosynthesis of tetrapyrrole compounds such as chlorophyll a 1 vitamin B12 and heme can be biosynthesized via two pathways the Shemin pathway C4 pathway 1-7 and the C5 pathway 8-14 Fig. 1 . In the Shemin pathway ALA 3 is biosynthesized by the condensation of glycine 4 and succinyl CoA 5 . In the C5 pathway ALA 3 is derived from all the carbons of L-glutamate L-glutamic acid 6 . Mayer et al. reported that ALA 3 is biosynthesized via the C5 pathway in Euglena gracilis 12 . Beale et al. reported that E. gracilis contains ALA synthase 15 implying that ALA 3 may also be synthesized via the Shemin pathway. Weinstein et al. 16 reported that the C5 pathway in the chloroplast and ALA synthase probably in the mitochondrion of E. gracilis operate simultaneously to biosynthesize ALA. They also showed that 2-14C glycine was incorporated .

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