tailieunhanh - Báo cáo Y học: Cloning and expression of sterol D14-reductase from bovine liver

Biosynthesis of cholesterol represents one of the funda-mental cellular metabolic processes. SterolD14-reductase (D14-SR) is a microsomal enzyme involved in the con-version of lanosterol to cholesterol in mammals. Amino-acid sequence analysis of a 38-kDa protein puri®ed from bovine liver in our laboratory revealed 90% similarity with a human sterol reductase, SR-1, encoded by the TM7SF2gene, and with the C-terminal domain of human lamin B receptor. | Eur. J. Biochem. 269 283-290 2002 FEBS 2002 Cloning and expression of sterol D14-reductase from bovine liver Rita Robert 1 Anna Maria Bennati1 Giovanni Galli2 Donatella Caruso2 Bruno Maras3 Cristina Aisa4 Tommaso Beccari4 Maria Agnese Della Fazia4 and Giuseppe Servillo4 1 Department of Internal Medicine University of Perugia Italy department of Pharmacological Sciences University of Milan Italy 3Department of Biochemical Sciences A. Rossi Fanelli Universita Ma Sapienza Roma Italy department of Biochemical Sciences and Molecular Biotechnology University of Perugia Italy Biosynthesis of cholesterol represents one of the fundamental cellular metabolic processes. Sterol A14-reductase A14-SR is a microsomal enzyme involved in the conversion of lanosterol to cholesterol in mammals. Aminoacid sequence analysis of a 38-kDa protein purihed from bovine liver in our laboratory revealed 90 similarity with a human sterol reductase SR-1 encoded by the TM7SF2 gene and with the C-terminal domain of human lamin B receptor. A cDNA encoding the 38-kDa protein similar to human TM7SF2 was identihed by analysis of a bovine expressed sequence tag EST database. The cDNA was synthesized by RT-PCR cloned and sequenced. The cDNA encodes a 418 amino-acid polypeptide with nine predicted transmembrane domains. The deduced amino-acid sequence exhibits high similarity with A14-SR from yeasts fungi and plants 55-59 suggesting that the bovine cDNA encodes A14-SR. Northern blot analysis of bovine tissues showed high expression of mRNA in liver and brain. The polypeptide encoded by the cloned cDNA was expressed in COS-7 cells. Immunofluorescence analysis of transfected cells revealed a distribution of the protein throughout the ER. COS-7 cells expressing the protein exhibited A14-SR activity about sevenfold higher than control cells. These results demonstrate that the cloned bovine cDNA encodes A14-SR and provide evidence that the human TM7SF2 gene encodes A14-SR. Keywords sterol biosynthesis sterol

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• medical knowledge
• medical research
• analysis of cytoplasmic
• BMC Plant Biology
• Upland cotton
• Restorer gene
• High throughput sequencing
• Cytoplasmic male sterility
• Plant Biology
• Multi omics analysis
• Brassica napus
• Naturally exists
• Mitochondrial genome functions
• BMC Genomics
• RNA sequencing
• Restorer of fertility
• Sterile phenotype
• Parental genes
• Binding miRNAs
• Triple hybrids
• Gossypium bickii
• Proteomic analysis
• biochemistry
• molecular Biology
• microRNA genes
• hemoglobin
• medical analysis
• environmental medicine
• human disease
• Crystal structure
• biochemical studies
• Gossypium hirsutum L
• Photosensitive genetic male sterile
• Differentially expressed genes
• Transcriptome analysis
• RNA Seq
• mammals
• tài liệu báo cáo nghiên cứu khoa học
• cách trình bày báo cáo
• kiến thức bệnh thú y
• báo cáo bệnh thú y
• các nghiên về bệnh thú ý
• scientific research
• Human telomeric
• Structural features
• hormone medicine
• under construction
• cytoplasm
• molecular
• biosynthesis
• genetics
• PPR protein
• Targeted mutagenesis
• Nuclear restorer gene
• Structure function relationship
• Rubber tree
• Hevea brasiliensis
• Genome sequencing
• Anther transcriptome
• Hybrid wheat
• Pistil like
• Skeletal muscle
• Wnt signaling pathway
• Enzyme activity
• Cytoplasmic surface
• Fertility restorer
• Plant breeders
• Nuclear genes