tailieunhanh - Ảnh hưởng của các chất điều hòa sinh trưởng thực vật lên sự phát sinh hình thái của mẫu cấy lát mỏng tế bào cuống chồi hoa Súp lơ

Kết quả nghiên cứu đã chỉ ra rằng việc lựa chọn các chất điều hòa sinh trưởng thực vật là yếu tố chính xác định dạng đáp ứng phát sinh hình thái của mẫu cấy TTCL từ cuống chồi hoa Súp lơ. | Tạp chí Công nghệ Sinh học 7 2 229-233 2009 EFFECTS OF PLANT GROWTH REGULATORS ON THE MORPHOGENESIS OF CAULIFLOWER CURD TRANSVERSE THIN CELL LAYER EXPLANTS Duong Tan Nhut1 Bui Van The Vinh2 Tay Nguyen Institute of Biology 2University of Technology Ho Chi Minh City SUMMARY Transverse thin cell layers tTCLs from mature curd of Cauliflower Brassica oleracea var. botrytis were cultured on Murashige and Skoog medium supplemented with different types of plant growth regulators PGRs at various concentrations. The results were recorded after 6 weeks of culture. The presence of 2 4-dichlorophenoxy-acetic acid 2 4-D at - mg r1 resulted in callus formation while roots formed in culture medium supplemented with - mg r1 NAA naphthalene acetic acid . When 1-phenyl-3- 1 2 3-thiadiazol-5-yl -urea TDZ was added to culture medium at - mg r1 shoot clusters regenerated directly from the edge of explants. Shoots initiated in the presence of TDZ but without stem elongation and leaf formation. In order to obtain normal plant development clumps of regenerated shoots were transferred onto PGR-free MS medium. Approximately 26 shoots with normal stem elongation developed from each tTCL after 2 weeks. Rooting was obtained by transferring shoots to MS medium supplemented with mg r1 NAA and mg T Kinetin. Regenerated plants with adventitious roots were transferred to soil. The results demonstrated that the choice of PGRs is of significance in determining the morphogenesis of cauliflower curd tTCL explants. Keywords Brassica oleracea var. botrytis curd TCL morphogenesis PGRs INTRODUCTION Cauliflower is one of the most important vegetable crops in the world because of theft nutritious value. According to the United States Department of Agriculture 100 g of raw cauliflower provides 77 of an adult s Dietary Reference Intakes DRI of vitamin c. It is also a source of dietary fiber vitamin B6 folate pantothenic acid as well as small amounts of other vitamins and .