tailieunhanh - Ebook Immunohematology and transfusion medicine - A case study approach: Part 2

(BQ) Part 2 book "Immunohematology and transfusion medicine - A case study approach" presents the following contents: Playing with enzymes, differential alloadsorption, the case of low platelets, cruising for a bruising, do the math,. | Chapter 14 Playing with Enzymes Clinical History A 70-year-old female of African American decent with history of hypertension type 2 diabetes mellitus anemia and congestive heart failure is admitted to the hospital because of symptomatic anemia hemoglobin Hgb level g dL . The patient has a history of red blood cell RBC transfusions and is known to have alloantibodies anti-C -K and -Fya . The patient was last transfused two units of RBCs 2 weeks ago. Two RBC units are now requested and a type and crossmatch sample ethylenediaminetetraacetic acid EDTA anticoagulant is submitted to the blood bank. ABO Rh Antibody Screen ABO Rh gel method Patient RBCs forward typing Patient plasma reverse typing Anti-A Anti-B Anti-D A1 cells B cells 0 0 4 4 4 Antibody screen gel method SC1 2 SC2 2 Reaction scale 0 no reaction to 4 strong reaction RBC red blood cell Crossmatched RBC s Group O C- K- Fya antigen Negative AHG cc Donor Unit 1 1 NT Donor Unit 2 2 NT Springer International Publishing Switzerland 2016 67 M. T. Friedman et al. Immunohematology and Transfusion Medicine DOI 978-3-319-22342-1 14 Gel and Enzyme Panel Selected Cells Rh-hr Kell Duffy Kidd Lewis MNS p Lutheran Test Results Cell Rh-hr D c E c e f C V K k Kp- Kp Jsa Jsb Fya Fyb Jk Jkb Lea Leb M N s s Pl Lua Lub Cell Gel Ficin 1 R2R2 0 0 0 0 0 0 0 0 0 0 0 1 1 1 2 RjRi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 3 RxRx 0 0 0 0 0 0 0 0 0 0 0 0 s 3 1 1 4 RoT 0 0 0 0 0 0 0 0 0 0 0 4 0 0 5 RoT 0 0 0 0 0 0 0 0 0 0 0 0 5 1 1 6 rr 0 0 0 0 0 0 0 0 0 0 0 0 w 0 6 1 1 7 rr 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s 0 7 1 1 8 RoT 0 0 0 0 0 0 0 0 0 0 0 0 s 0 8 2 0 9 rr 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9 1 1 10 RoT 0 0 0 0 0 0 0 0 0 0 0 w 0 10 1 1 Patient Cell w NT Reaction scale 0 no reaction to 4 strong reaction DAT Profile Polyspeciflc w IgG w Cjd 0 Playing with Enzymes Acid Eluate Panel Rh-hr Kell Duffy Kidd Lewis MNS p Lutheran Test Results lAT Tube Cell Rh-hr D c E c e f cw V K k Kp- Kp Jsa Jsb Fya Fy Jka Jkb Lea Leb M N s s Pl Lua Lub Cell .