tailieunhanh - Báo cáo khoa học: Infrared spectroscopy as a tool for discrimination between sensitive and multiresistant K562 cells

Fourier transform infrared spectroscopy was performed on human leukemic daunorubicin-sensitiveK562 cells and their multiresistant counterpart derived by selection. Statistical analysis, including variable reduction and linear discrimi-nant analysis was performed on sensitive and multiresistant cells spectra in order to establish a diagnostic tool for multiresistant pattern. For each of the twomethods of data reduction tested [genetic algorithm or principal component analysis (PCA)] discriminationbetween the twocell lineswas found to be possible | Eur. J. Biochem. 269 1968-1973 2002 FEBS 2002 doi Infrared spectroscopy as a tool for discrimination between sensitive and multiresistant K562 cells Anthoula Gaigneaux Jean-Marie Ruysschaert and Erik Goormaghtigh Laboratory of Structure and Function of Biological Membranes Free University of Brussels Belgium Fourier transform infrared spectroscopy was performed on human leukemic daunorubicin-sensitive K562 cells and their multiresistant counterpart derived by selection. Statistical analysis including variable reduction and linear discriminant analysis was performed on sensitive and multiresistant cells spectra in order to establish a diagnostic tool for multiresistant pattern. For each of the two methods of data reduction tested genetic algorithm or principal component analysis PCA discrimination between the two cell lines was found to be possible. The best results obtained with PCA-reduction showed an accuracy of 93 on a distinct test set of spectra. These results demonstrate the efficiency of Fourier transform infrared spectroscopy for classification. Further analysis of the spectral differences indicated that discrimination between resistant and sensitive cells was based on variations in all cellular contents. Lipid and nucleic acid decreased relatively while the protein content increased. Keywords multiresistance infrared spectroscopy multivariate statistics K562 leukemia. In recent years infrared spectroscopy has been a powerful tool for biodiagnostics 1 . A major advantage of infrared spectroscopy over more classical techniques of investigation is that neither staining of the samples nor chemical reagent additions are necessary. Just a few minutes and a few 1L of a cell suspension are sufficient to obtain a spectrum representative of all cell constituents. This technique is based on absorption of infrared light by the vibrational transitions in covalent bonds. Intensities provide quantitative information while frequencies give