tailieunhanh - Molecular Biotechnology-Lession 2: Recombinant DNA technology

Glofish is the first transgenic animal approved to be consumed by human in the USA The insertion of different constructs of GFP into the fish genomes to give different green idea for recombinant DNA was first proposed by Peter Lobban, a graduate student of Prof. Dale Kaiser in the Biochemistry Department at Stanford University Medical School. The first publications describing the successful production and intracellular replication of recombinant DNA appeared in 1972 and 1973. Stanford University applied for a US patent on recombinant DNA in 1974, listing the inventors as Stanley N. Cohen and Herbert W. Boyer; this patent was awarded in 1980. The first licensed drug generated using recombinant DNA technology was human insulin, developed by. | Recombinant DNA technology (Gene cloning, genetic engineering) VIETNAM NATIONAL UNIVERSITY AT HO CHI MINH CITY INTERNATIONAL UNIVERSITY SCHOOL OF BIOTECHNOLOGY Tran Ngoc Duc, PhD 2. Prokaryotic and eukaryotic genes 3. Vectors 4. Cloning a gene 5. Transformation technology 6. Detection of the cloned gene/products 1. Introduction 1/6 Introduction Glofish is the first transgenic animal approved to be consumed by human in the USA The insertion of different constructs of GFP into the fish genomes to give different green colors The idea for recombinant DNA was first proposed by Peter Lobban, a graduate student of Prof. Dale Kaiser in the Biochemistry Department at Stanford University Medical School. The first publications describing the successful production and intracellular replication of recombinant DNA appeared in 1972 and 1973. Stanford University applied for a US patent on recombinant DNA in 1974, listing the inventors as Stanley N. Cohen and Herbert W. Boyer; this patent was . | Recombinant DNA technology (Gene cloning, genetic engineering) VIETNAM NATIONAL UNIVERSITY AT HO CHI MINH CITY INTERNATIONAL UNIVERSITY SCHOOL OF BIOTECHNOLOGY Tran Ngoc Duc, PhD 2. Prokaryotic and eukaryotic genes 3. Vectors 4. Cloning a gene 5. Transformation technology 6. Detection of the cloned gene/products 1. Introduction 1/6 Introduction Glofish is the first transgenic animal approved to be consumed by human in the USA The insertion of different constructs of GFP into the fish genomes to give different green colors The idea for recombinant DNA was first proposed by Peter Lobban, a graduate student of Prof. Dale Kaiser in the Biochemistry Department at Stanford University Medical School. The first publications describing the successful production and intracellular replication of recombinant DNA appeared in 1972 and 1973. Stanford University applied for a US patent on recombinant DNA in 1974, listing the inventors as Stanley N. Cohen and Herbert W. Boyer; this patent was awarded in 1980. The first licensed drug generated using recombinant DNA technology was human insulin, developed by Genentech and Licensed by Eli Lilly and Company () Applications of recombinant biotechnology Recombinant human insulin Recombinant human growth hormone Recombinant human blood clotting factor III Recombinant hepatitis B vaccine Golden rice Herbicide resistant crop Insecticide resistant crop 2/6 Prokaryotic and eukaryotic genes Prokaryotic Most prokaryote require only one single RNA polymerase for all types, which is different from eukaryote requiring 3 different RNA polymerase Genes are usually found grouped together in operons, which are group of related genes for a metabolic pathway under a single promoter (regulatory region) lacZ encodes β-galactosidase (LacZ), an intracellular enzyme that cleaves the disaccharide lactose into glucose and galactose. lacY encodes β-galactoside permease (LacY), a membrane-bound transport .