tailieunhanh - Báo cáo khoa học: Cellular retinol-binding protein type II (CRBPII) in adult zebrafish (Danio rerio) cDNA sequence, tissue-specific expression and gene linkage analysis

We have determined the nucleotide sequence of a zebrafish cDNAclone that codes for a cellular retinol-binding protein type II (CRBPII). Radiation hybrid mapping revealed that the zebrafish and human CRBPII genes are located in syntenic situhybridization and emulsion autora-diography localized theCRBPIImRNA to the intestine and the liver of adult zebrafish. | Eur. J. Biochem. 269 4685-4692 2002 FEBS 2002 doi Cellular retinol-binding protein type II CRBPII in adult zebrafish Danio rerio cDNA sequence tissue-specific expression and gene linkage analysis Marianne C. Cameron1 Eileen M. Denovan-Wright2 Mukesh K. Sharma1 and Jonathan M. Wright1 1 Department of Biology and department of Pharmacology Dalhousie University Halifax Nova Scotia Canada We have determined the nucleotide sequence of a zebrafish cDNA clone that codes for a cellular retinol-binding protein type II CRBPII . Radiation hybrid mapping revealed that the zebrafish and human CRBPII genes are located in syntenic groups. In situ hybridization and emulsion autoradiography localized the CRBPII mRNA to the intestine and the liver of adult zebrafish. CRBPII and intestinal fatty acid binding protein I-FABP mRNA was colocalized to the same regions along the anterior-posterior gradient of the zebrafish intestine. Similarly CRBPII and I-FABP mRNA are colocalized in mammalian and chicken intestine. CRBPII mRNA but not I-FABP mRNA was detected in adult zebrafish liver which is in contrast to mammals where liver CRBPII mRNA levels are high during development but rapidly decrease to very low or undetectable levels following birth. CRBPII and I-FABP gene expression appears there fore to be co-ordinately regulated in the zebrafish intestine as has been suggested for mammals and chicken but CRBPII gene expression is markedly different in the liver of adult zebrafish compared to the livers of mammals. As such retinol metabolism in zebrafish may differ from that of mammals and require continued production of CRBPII in adult liver. The primary sequence of the coding regions of fish and mammalian CRBPII genes their relative chromosomal location in syntenic groups and possibly portions of the control regions involved in regulation of CRBPII gene expression in the intestine appear therefore to have been conserved for more than 400 million years. .

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