tailieunhanh - Production of transgenic deepwater indica rice plants expressing a synthetic Bacillus thuringiensis cryIA(b) gene with enhanced resistance to yellow stem borer

Yellow stem borer has been identified as a major insect pest of deepwater rice, causing severe yield losses. Bt gene(s) from Bacillus thuringiensis have been proven very effective in pest resistance program. The use of transgenic plants expressing Bt gene(s) is now occupied effective approach to control insect infestation. We have successfully introduced a synthetic cryIA(b) gene into embryogenic calli of a deepwater indica rice variety, Vaidehi, by using the biolistic method of transformation. The presence and expression of the Bt gene in regenerated plants were confirmed by Southern and Western blot analyses. Inheritance of the transgene was confirmed in the T1 generation. Insect bioassays showed. | ELSEVIER Plant Science 135 1998 25-30 Production of transgenic deepwater indica rice plants expressing a synthetic Bacillus thuringiensis cryIA b gene with enhanced resistance to yellow stem borer Mohammad Firoz Alam Karabi Datta Editha Abrigo Alelie Vasquez Dharmawansa Senadhira Swapan K. Datta Plant Breeding Genetics and Biochemistry Division International Rice Research Institute . Box 933 Manila 1099 Philippines Received 14 October 1997 received in revised form 17 December 1997 accepted 23 February 1998 Abstract Yellow stem borer has been identified as a major insect pest of deepwater rice causing severe yield losses. Bt gene s from Bacillus thuringiensis have been proven very effective in pest resistance program. The use of transgenic plants expressing Bt gene s is now occupied effective approach to control insect infestation. We have successfully introduced a synthetic cryIA b gene into embryogenic calli of a deepwater indica rice variety Vaidehi by using the biolistic method of transformation. The presence and expression of the Bt gene in regenerated plants were confirmed by Southern and Western blot analyses. Inheritance of the transgene was confirmed in the T1 generation. Insect bioassays showed an enhancement of resistance against the yellow stem borer. This is the first report of lowland rice engineered with Bt gene. 1998 Elsevier Science Ireland Ltd. All rights reserved. Keywords Transgenic deepwater rice cryIA b gene Yellow stem borer resistance 1. Introduction Deepwater rice DWR is grown on about 9 million hectares of flooded lands in Asia the river Abbreviations 2 4-D 2 4-Dichlorophenoxy acetic acid NAA 1-a Napthalene acetic acid. Corresponding author. Tel. 63 2 8450563 8450569 fax 63 2 7612406 8450606 e-mail sdatta@ basins of Ganges-Brahmaputra of India and Bangladesh the Irrawaddy of Myanmar the Mekong of Vietnam and Cambodia and the Chao Phraya of Thailand and West Africa upper and middle basins of the Niger River 1 . Farmers in .