tailieunhanh - Regeneration and Transformation via Agrobacterium tumefaciens of Echinacea purpurea L.

Leaf explants of Echinacea purpurea L. taken from aseptically germinated seedlings were inoculated with A. tumefaciens strains EHA105, carrying a binary vector conferring herbicide resistant bar gene and fungal resistant chitinase gene. Glufosinate ammonium‐resistant shoots were regenerated on a medium containing BAP and NAA at a concentration of and μM, respectively. A subsequent transfer of shoots to medium containing BAP was necessary for stem elongation and leaf development. Transgenic Echinacea plants carrying bar and chitinase genes were selected for their resistance to glufosinate ammonium herbicide. Molecular analysis using PCR confirmed the integration of the transgenes into plant genome. This is the first report on genetic transformation of Echinacea. | Plant Tissue Cult. Biotech. 20 2 101-111 2010 December PTC B Regeneration and Transformation via Agrobacterium tumefaciens of Echinacea purpurea L. Moemen Hanafy1 Usama I. Aly and Mohamed A. Matter Plant Biotechnology Department National Research Centre El-Behooth Str. Dokki Cairo Egypt Key words Echinacea purpurea Regeneration A. tumefaciens Transformation Abstract Leaf explants of Echinacea purpurea L. taken from aseptically germinated seedlings were inoculated with A. tumefaciens strains EHA105 carrying a binary vector conferring herbicide resistant bar gene and fungal resistant chitinase gene. Glufosinate ammonium-resistant shoots were regenerated on a medium containing BAP and NAA at a concentration of and pM respectively. A subsequent transfer of shoots to medium containing BAP was necessary for stem elongation and leaf development. Transgenic Echinacea plants carrying bar and chitinase genes were selected for their resistance to glufosinate ammonium herbicide. Molecular analysis using PCR confirmed the integration of the transgenes into plant genome. This is the first report on genetic transformation of Echinacea plant using bar gene as a selectable marker. Introduction Echinacea purpurea L. is a group of purple coneflowers in the family Asteraceae. It has been used traditionally as an herbal medicine and dietary supplements for hundreds of years Percival 2000 . In recent years the purple coneflower has gained global attractiveness due to it s beneficially effect on human s immune system Bauer and Wagner 1991 . Extracts from the plant have shown antioxidative antibacterial antiviral and antifungal properties and are used in the treatment of the common cold as well as respiratory and urinary diseases Grimm and Muller 1999 Barrett 2003 . Recent technological advances have allowed researchers to analyze some of the medicinally active compounds present in Echinacea sp. and to speculate on their modes of action Choffe et al. 2000 . Complex .

• Báo cáo khoa học
• Echinacea purpurea
• Regeneration
• A
• tumefaciens
• Transformation
• Plant Biotechnology Department
• Antioxidant content
• Cultivated sunflower
• Fatty acid
• Helianthus annuus
• Wide hybridization
• Agricultural science
• Biochemical changes
• Growth parameters in Echinacea purpurea callus
• Suspension culture
• Cell suspension
• báo cáo khoa học
• báo cáo y học
• công trình nghiên cứu về y học
• tài liệu về y học
• cách trình bày báo cáo
• tài liệu báo cáo nghiên cứu khoa học
• kiến thức bệnh thú y
• báo cáo bệnh thú y
• các nghiên về bệnh thú ý