tailieunhanh - Molecular identification of mango malformation pathogens in Egypt

Diagnostic tests by molecular biology is made for studying the relations among Fusarium species for linking production of proteins, degree of relationship and occurrence of malformation. Determination of proteins for isolates causing-disease by SDS-PAGE explained there’s specific band for each fungus and there are common bands among some isolates of fungi. Since, band with MW 30 KDa represented only in F. proliferatum and F. oxysporum and F. subgluti-nans respectively. This band considered as specific band for these isolates, which released high patho-genisity effect. RAPD-PCR markers were used to discriminate variations between Fusarium isolates and causing disease. There is specific band. | Advances in Bioscience and Biotechnology 2011 2 226-232 doi Published Online August 2011 http journal abb . ABB Molecular identification of mango malformation pathogens in Egypt Wafaa Mohamed Haggag1 Mahmoud Hazza2 Mahmoud Saker3 Mohamed Abd El-Wahab1 1Department of Plant Pathology National Research Center Cairo Egypt 2Science Faculty Botany Department Banha University Banha Egypt 3Department of Plant Biotechnology National Research Center Cairo Egypt. Email wafaa haggag@ Received 16 February 2011 revised 21 May 2011 accepted 7 June 2011. ABSTRACT Diagnostic tests by molecular biology is made for studying the relations among Fusarium species for linking production of proteins degree of relationship and occurrence of malformation. Determination of proteins for isolates causing-disease by SDS-PAGE explained there s specific band for each fungus and there are common bands among some isolates of fungi. Since band with MW 30 KDa represented only in F. proliferatum and F. oxysporum and F. subgluti-nans respectively. This band considered as specific band for these isolates which released high patho-genisity effect. RAPD-PCR markers were used to discriminate variations between Fusarium isolates and causing disease. There is specific band for each fungus which act as molecular marker for each fungus and there are some bands common among some isolates of pathogenic fungi. The dendrogram shows there is degree of relationship between F. sterilihy-phosum and F. proliferatum between F. moniliforme and F. subglutinans between F. oxysporum and F. chlamydospore the degree of relationship among F. subglutinans F. proliferatum and F. sterilihyphosum and degree of relationship among F. moniliforme F. sterilihyphosum F. proliferatum and F. subglutinans Keywords Fusarium Spp. Mango Malformation 1. INTRODUCTION Mango Mangifera indica L. is the most important fruit grown in tropical and subtropical region of the world. Mango is the most .

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