tailieunhanh - Báo cáo Y học: Cloning and characterization of the mammalian-specific nicolin 1 gene (NICN1) encoding a nuclear 24 kDa protein

Wehave identifiedanovelmammaliangene, termednicolin1 gene (NICN1), that is present in human, dog and mouse, whereas it is absent from the available genome sequences of nonmammalian organisms. TheNICN1gene consists of six exons and spans about 6 kb of genomic DNA. It encodes a 213 amino acid protein that does not belong to any known protein family. Experiments using green fluorescent protein (GFP)-taggednicolin1 fusionproteins indicate that nicolin1 is a nuclear protein. | Eur. J. Biochem. 269 5240-5245 2002 FEBS 2002 doi Cloning and characterization of the mammalian-specific nicolin 1 gene NICN1 encoding a nuclear 24 kDa protein Bianca Backofen1 I Ralf Jacob2 Katrin Serth3 Achim Gossler3 Hassan Y. Naim2 and Tosso Leeb1 1 Institute of Animal Breeding and Genetics and 2Institute of Physiological Chemistry School of Veterinary Medicine Hannover 3Institute of Molecular Biology Medical School Hannover Hannover Germany We have identified a novel mammalian gene termed nicolin 1 gene NICN1 that is present in human dog and mouse whereas it is absent from the available genome sequences of nonmammalian organisms. The NICN1 gene consists of six exons and spans about 6 kb of genomic DNA. It encodes a 213 amino acid protein that does not belong to any known protein family. Experiments using green fluorescent protein GFP -tagged nicolin 1 fusion proteins indicate that nicolin 1 is a nuclear protein. Northern analysis and semiquantitative RT-PCR demonstrated that the kb NICN1 mRNA is expressed in a tissue-specific manner. The highest NICN1 expression levels are found in brain testis liver and kidney. On the other hand the NICN1 expression is weak in spleen leukocytes small intestine and colon. The NICN1 gene is also expressed during development. Keywords nicolin comparative genomics human mouse dog. The initial completion of the human genome sequence revealed that the function of only about 15 000 of the estimated 35 000 genes in the human genome is known 1 . Systematic approaches have been undertaken to identify expressed sequences and full length cDNAs in several model organisms which generated a wealth of sequence information on previously unidentified genes 2 . However at present a most immediate task is the functional characterization of all the newly identified genes. In contrast to genomic and cDNA sequencing no universal high-throughput parallel approach is currently available to functionally .

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