tailieunhanh - Báo cáo khoa học: The lectin from leaves of Japanese cycad, Cycas revoluta Thunb. (gymnosperm) is a member of the jacalin-related family

A novel lectin was isolated from leaves of the Japanese cycad,Cycas revolutaThunb. (gymnosperm), and its char-acteristics including amino acid composition, molecular mass, carbohydrate binding specificity and partial amino acid sequences were examined. The inhibition analysis of hemagglutinating activity with various sugars showed that the lectin has a carbohydrate-binding specificity similar to those of mannose recognizing, jacalin-related lectins. Partial amino acid sequences of the lysylendopeptic peptides shows that the lectinmight have a repeating structure andbelong to the jacalin-related lectin family | Eur. J. Biochem. 269 4335-4341 2002 FEBS 2002 doi The lectin from leaves of Japanese cycad Cycas revoluta Thunb. gymnosperm is a member of the jacalin-related family Fumio Yagi1 2 Toshinobu Iwaya1 Tomokazu Haraguchi1 and Irwin J. Goldstein2 1 Department of Biochemical Science and Technology Faculty of Agriculture Kagoshima University Kagoshima Japan department of Biological Chemistry Medical School The University of Michigan Ann Arbor Michigan USA A novel lectin was isolated from leaves of the Japanese cycad Cycas revoluta Thunb. gymnosperm and its characteristics including amino acid composition molecular mass carbohydrate binding specificity and partial amino acid sequences were examined. The inhibition analysis of hemagglutinating activity with various sugars showed that the lectin has a carbohydrate-binding specificity similar to those of mannose recognizing jacalin-related lectins. Partial amino acid sequences of the lysylendopeptic peptides shows that the lectin might have a repeating structure and belong to the jacalin-related lectin family. Keywords Cycas revoluta Thunb. gymnosperm Japanese cycad leaf lectin. Lectins have been isolated from many angiospermous plants including legumes and monocots 1 2 . Many applications have been found for their use in biological and bimedical research 3 . It has been suggested that plant lectins be classified into seven different protein families 4 . Thus far only two isolectins have been isolated from seeds of a gymnospermous plant that of Brazilian cedar belonging to the family of Coniferae 5 . Except for these seed lectins no lectins of gymnosperm origin have been purified nor has the relationship between angiosperms and gymnosperms been established. Previously Pettitt 6 reported hemagglutinating activity in the extracts from cycad pollen. Until now no other reports have appeared on lectin activity in other parts of cycad plants. This paper deals with the isolation and characterization .

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