tailieunhanh - Báo cáo khoa học: Amyloidogenic nature of spider silk

In spiders soluble proteins are converted to form insoluble silk fibres, stronger than steel. The final fibre product has longbeen the subject of study;however, little is knownabout the conversion process in the silk-producing gland of the spider. Here we describe a study of the conversion of the soluble form of the major spider-silk protein, spidroin, directly extracted from the silk gland, to ab-sheet enriched stateusing circular dichroism(CD) spectroscopy. | Eur. J. Biochem. 269 4159-4163 2002 FEBS 2002 doi Amyloidogenic nature of spider silk John M. Kenney1 David Knight2 Michael J. Wise3 and Fritz Vollrath2 4 Institute for Storage Ring Facilities University of Aarhus Denmark 2Department of Zoology University of Oxford UK 3Department of Genetics University of Cambridge UK 4Department of Zoology University of Aarhus Denmark In spiders soluble proteins are converted to form insoluble silk fibres stronger than steel. The final fibre product has long been the subject of study however line is known about the conversion process in the silk-producing gland of the spider. Here we describe a study of the conversion of the soluble form of the major spider-silk protein spidroin directly extracted from the silk gland to a b-sheet enriched state using circular dichroism CD spectroscopy. Combined with electron microscopy EM data showing fibril formation in the b-sheet rich region of the gland and amino-acid sequence analyses linking spidroin and amyloids these results lead us to suggest that the refolding conversion is amyloid like. We also propose that spider silk could be a valuable model system for testing hypotheses concerning b-sheet formation in other fibrilogenic systems including amyloids. Keywords spider silk spidrom amyloids CD spectloscopy low complexity peptides. Spiders have evolved sophisticated systems not only to produce and store proteins at high concentrations 30 in solution but also to control the conversion of these soluble proteins to form insoluble silk fibres 1 . The conversion process appears to rely on refolding of one or two members of the spidroin family of major silk proteins 2 3 but is poorly understood. Although the structure of the insect silkworm Bombyx mori silk has been well characterized 4 and a model 5 has been proposed for insect silk formation based on regenerated fibroin the major protein in silkworm silk the structure of spider silk is less well understood and

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