tailieunhanh - Báo cáo khoa học: Enhancement of oxidative stress-induced apoptosis by Hsp105a in mouse embryonal F9 cells

Hsp105aisoneof themajormammalianheat shockproteins that belongs to the HSP105/110 family, and is expressed at especially high levels in the brain as compared with other tissues in mammals. Previously, we showed that Hsp105a prevents stress-induced apoptosis in neuronal PC12 cells, and is a novel anti-apoptotic neuroprotective factor in the mammalian brain. | Eur. J. Biochem. 269 4143-4151 2002 FEBS 2002 doi Enhancement of oxidative stress-induced apoptosis by Hsp105a in mouse embryonal F9 cells Nobuyuki Yamagishi Youhei Saito Keiichi Ishihara and Takumi Hatayama Department of Biochemistry Kyoto Pharmaceutical University Japan Hsp105a is one of the major mammalian heat shock proteins that belongs to the HSP105 110 family and is expressed at especially high levels in the brain as compared with other tissues in mammals. Previously we showed that Hsp105a prevents stress-induced apoptosis in neuronal PC12 cells and is a novel anti-apoptotic neuroprotective factor in the mammalian brain. On the other hand we have also demonstrated that Hsp105a is expressed transiently at high levels during mouse embryogenesis and is found not only in various tissues but also in apoptotic cells. In the present study to elucidate the role of Hsp105a during mouse embryogenesis we established mouse embryonal F9 cell lines that constitutively over-express Hsp105a. Overexpression of Hsp105a enhanced hydrogen peroxide induced apoptosis by enhancing the activation of caspase-3 poly ADP-ribose polymerase cleavage cytochrome c release and activation of p38 mitogen-activated protein kinase p38 . Furthermore oxidative stress-induced apoptosis was suppressed by SB202190 a potent inhibitor of p38 in F9 cells. These findings indicated that the activation of p38 is an essential step for apoptosis in F9 cells and that Hsp105a enhances activation of p38 release of cytochrome c and caspase activation. Hsp105a may play important roles in organogenesis during which marked apoptosis occurs by enhancing apoptosis during mouse embryogenesis. Keywords apoptosis F9 cells Hsp105 p38 MAPK oxidative stress. Cell death is classified into two major morphologically and biochemically distinct modes necrosis and apoptosis 1 . Necrosis is characterized by swelling of organelles and cells followed by lysis of the plasma membrane and random DNA

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• tumor cells
• medical knowledge
• chemical reaction
• Elements of Chemical Reaction Engineering
• Chemical Reaction Engineering
• Hóa học và ứng dụng
• Kỹ thuật hóa học
• Phản ứng hóa học
• Kỹ thuật phản ứng hóa học
• The chemical vapor deposition condition
• The electrochemically catalytic efficiency
• Hydrogen evolution reaction in MoS2 nanoparticles
• Hydrogen evolution reaction
• Metal organic chemical vapor deposition
• Reaction Kinetics
• Heterogeneous Catalysis
• Elementary Reactions
• Microkinetic Analysis
• Nonisothermal Reactors
• Catalytic Reactions
• Quantum chemical modeling mechanism
• Semiempirical method RM1
• Quantum chemical study
• Half chair shape
• Diels Alder reaction
• Chemical reaction engirneering
• Hoá phân tích
• Giải pháp phản ứng hóa học
• Radiation on MHD casson fluid flow
• Chemical reaction effects on MHD casson fluid flow
• Porous medium with suction injection
• The occurrence of suction/injection
• The central PDEs
• Radiation of MHD visco elastic fluid flow over
• Chemical reaction on heat of MHD visco elastic
• Mass transfer of MHD visco elastic fluid flow
• Exponentially stretching sheet through porous medium
• Radiation on the boundary layer MHD casson fluid
• Chemical reaction effects on the boundary layer MHD casson fluid
• Vertical plate embedded in the porous medium
• Casson fluid flow free convection boundary layer fl
• Extreme learning machine
• Artificial chemical reaction optimization algorithm
• Single hidden layer feed forward neural network
• Learning algorithm
• Chemical reaction kinetic
• Adsorption kinetics
• Pseudo first order model
• Pseudo second order model
• General kinetic model
• Dried Probiotics
• Industrial Processes
• Membrane Separation
• Wastewater
• Maillard Reaction
• Pros and Cons
• Chemical kinetics
• Reactant concentration
• Reaction rates and stoichiometry
• Action of catalysts
• Surface area
• Chemical kinetics
• Introduction to general
• Chemical bonds
• Chemical reactions
• Reaction rates
• Chemical equilibrium
• Nuclear chemistry
• Nuclear engineering and technology
• Correlation between rare earth elements
• The chemical interactions of HT9 cladding
• FueleCladding Chemical Interaction
• Higher reaction thickness
• Summary of Chemical PhD Thesis
• Theoretical and Physical Chemistry
• CO hydrogenation reaction
• Ni Cu bimetallic catalyst system
• Density functional method
• Chemical method
• Lecture General Chemistry 1
• Bài giảng Hóa học đại cương 1
• Balance chemical equations
• Calculate the percent yield of a reaction
• Chemical formulas
• Chemical equations
• organic chemistry
• hóa hữu cơ
• công nghệ hóa học
• Chemical Engineering
• common reaction mechanisms
• Chromatography in the petroleum industry
• oxidation
• BMC Bioinformatics
• Flux balance analysis
• Linear programming
• Design of metabolic network
• Constraint based model
• Growth rate
• Production rate
• Smaller reaction network
• Luận án Tiến sĩ
• Physical chemistry
• Fabbricated electrocatalysts
• Oxygen evolution reaction
• Proton exchange membrane
• breast cancer
• medicine
• cytotoxicity
• Atomic nucleus
• electrons
• Chemical compounds
• Electrochemistry