tailieunhanh - Báo cáo khoa học: Pherokine-2 and -3 Two Drosophila molecules related to pheromone/odor-binding proteins induced by viral and bacterial infections

Drosophilais a powerful model system to study the regula-tory and effector mechanisms of innate immunity. To iden-tify molecules induced in the course of viral infection in this insect, we have developed a model based on intrathoracic injection of the picorna-like DrosophilaCvirus(DCV).We have used MALDI-TOF mass spectrometry to compare the hemolymph of DCV infected flies and control flies. By contrast with the strong humoral response triggered by injectionof bacteriaor fungal spores, we have identifiedonly one molecule induced in the hemolymph of virus infected flies | Eur. J. Biochem. 270 3398-3407 2003 FEBS 2003 doi Pherokine-2 and -3 Two Drosophila molecules related to pheromone odor-binding proteins induced by viral and bacterial infections Laurence Sabatier 1 Emmanuelle Jouanauv1 t Catherine Dostert1 Daniel Zachary1. Jean-Luc Dimarca2. Philippe Bulet14 and Jean-Luc Imler1 1CNRS UPR9022 Institut de Biologie Moléculữire et Cellulaire Strasbourg France 2Entomed SA Illkirch France Drosophila is a powerful model system to study the regulatory and effector mechanisms of innate immunity. To identify molecules induced in the course of viral infection in this insect we have developed a model based on intrathoracic injection of the picorna-like Drosophila C virus D CV. We have used MALDI-TOF mass spectrometry to compare the hemolymph of DCV infected flies and control flies. By contrast with the strong humoral response triggered by injection of bacteria or fungal spores we have identified only one molecule induced in the hemolymph of virus infected flies. This molecule pherokine-2 Phk-2 is related to OS-D A10 Phk-1 which was previously characterized as a putative odor pheromone binding protein specifically expressed in antennae. The virus-induced molecule is also similar to the product of the gene CG9358 Phk-3 which is induced by septic injury. Both Phk-2 and Phk-3 are strongly expressed during metamorphosis suggesting that they may participate in tissue-remodeling. Keywords host-defense antiviral Drosophila C virus odorbinding protein tissue remodelling. Innate immunity enables multicellular organisms to detect and fight infectious microbes. In vertebrates the innate immune system also participates in the induction and shaping of the subsequent adaptive immune response carried by lymphocytes. The innate immune system involves pattern recognition receptors PRRs which recognize conserved molecular patterns from broad classes of microorganisms such as lipopolysaccharide LPS from Gram-negative bacteria .