tailieunhanh - Báo cáo khoa học: Calcium-dependent protein–protein interactions induce changes in proximity relationships of Cys48 and Cys64 in chicken skeletal troponin I

The goal of this study was to relate conformational changes in the N-terminal domain of chicken troponin I (TnI) to Ca 2+ activation of the actin–myosin two cysteine residues in this region (Cys48 and Cys64) were labeled with two sulfhydryl-reactive pyrene-containing fluorophores [N-(1-pyrene)maleimide, and N-(1-pyrene)-iodoacetamide].The labeled TnI showed a typical fluores-cence spectrum: two sharp peaks of monomer fluorescence and a broad peak of excimer fluorescence arising from the formation of an excited dimer (excimer) | Eur. J. Biochem. 270 3092-3100 2003 FEBS 2003 doi Calcium-dependent protein-protein interactions induce changes in proximity relationships of Cys48 and Cys64 in chicken skeletal troponin I Ying-Ming Liou and Ming-Wei Chen Department of Life Science Institute of Biochemistry National Chung-Hsing University Taichung Taiwan The goal of this study was to relate conformational changes in the N-terminal domain of chicken troponin I TnI to Ca2 activation of the actin-myosin interaction. The two cysteine residues in this region Cys48 and Cys64 were labeled with two sulfhydryl-reactive pyrene-containing fluorophores N- 1-pyrene maleimide and N- 1-pyrene -iodoacetamide . Tire labeled Tnl show-ed a ty iical fluorescence spectrum two sharp peaks of monomer fluorescence and a broad peak of excimer fluorescence arising from the formation of an excited dimer excimer . Rsullte ohtanred show that forming a binary complex of labeled TnI with skeletal TnC sTnC in the absence of Ca2 decreases the excimer fluorescence indicating a separation of the two reduction in excimer fluorescence does not occur when labeled TnI is complexed with cardiac TnC cTnC . TSc kitin cuusk OTÚy aaráal ectiaarinn o ì iSc Ca2 -dependent myohbrillar ATPase. The binding of Ca2 to the two N-teaminal sites of sTnC causes a signihcant decrease in excimer fluorescence and an increase in monomer fluorescence in complexes of labeled TnI with skeletal TnC or TnC TnT while Ca2 binding to site II of cTnC only causes an increase in monomer fluorescence but no change in excimer fluoresesnes. Thus a ccmlormaiional change m the N-teaminal region of TnI may be necessary for full activation of muscle contraction. Keywords proximity relationships cysteine residues pyrene-containing fluorophores monomer fluorescence excimer fluorescence. Ca2 activation of striated muscle is mediated by the troponin complex. Troponin is composed of three subunits troponin C TnC a Ca2 binding .

• Báo cáo khoa học
• scientific reports
• Report medicine
• traditional medicine
• tumor cells
• medical knowledge
• chemical reaction
• Elements of Chemical Reaction Engineering
• Chemical Reaction Engineering
• Hóa học và ứng dụng
• Kỹ thuật hóa học
• Phản ứng hóa học
• Kỹ thuật phản ứng hóa học
• The chemical vapor deposition condition
• The electrochemically catalytic efficiency
• Hydrogen evolution reaction in MoS2 nanoparticles
• Hydrogen evolution reaction
• Metal organic chemical vapor deposition
• Reaction Kinetics
• Heterogeneous Catalysis
• Elementary Reactions
• Microkinetic Analysis
• Nonisothermal Reactors
• Catalytic Reactions
• Quantum chemical modeling mechanism
• Semiempirical method RM1
• Quantum chemical study
• Half chair shape
• Diels Alder reaction
• Chemical reaction engirneering
• Hoá phân tích
• Giải pháp phản ứng hóa học
• Radiation on MHD casson fluid flow
• Chemical reaction effects on MHD casson fluid flow
• Porous medium with suction injection
• The occurrence of suction/injection
• The central PDEs
• Radiation of MHD visco elastic fluid flow over
• Chemical reaction on heat of MHD visco elastic
• Mass transfer of MHD visco elastic fluid flow
• Exponentially stretching sheet through porous medium
• Radiation on the boundary layer MHD casson fluid
• Chemical reaction effects on the boundary layer MHD casson fluid
• Vertical plate embedded in the porous medium
• Casson fluid flow free convection boundary layer fl
• Extreme learning machine
• Artificial chemical reaction optimization algorithm
• Single hidden layer feed forward neural network
• Learning algorithm
• Chemical reaction kinetic
• Adsorption kinetics
• Pseudo first order model
• Pseudo second order model
• General kinetic model
• Dried Probiotics
• Industrial Processes
• Membrane Separation
• Wastewater
• Maillard Reaction
• Pros and Cons
• Chemical kinetics
• Reactant concentration
• Reaction rates and stoichiometry
• Action of catalysts
• Surface area
• Chemical kinetics
• Introduction to general
• Chemical bonds
• Chemical reactions
• Reaction rates
• Chemical equilibrium
• Nuclear chemistry
• Nuclear engineering and technology
• Correlation between rare earth elements
• The chemical interactions of HT9 cladding
• FueleCladding Chemical Interaction
• Higher reaction thickness
• Summary of Chemical PhD Thesis
• Theoretical and Physical Chemistry
• CO hydrogenation reaction
• Ni Cu bimetallic catalyst system
• Density functional method
• Chemical method
• Lecture General Chemistry 1
• Bài giảng Hóa học đại cương 1
• Balance chemical equations
• Calculate the percent yield of a reaction
• Chemical formulas
• Chemical equations
• organic chemistry
• hóa hữu cơ
• công nghệ hóa học
• Chemical Engineering
• common reaction mechanisms
• Chromatography in the petroleum industry
• oxidation
• BMC Bioinformatics
• Flux balance analysis
• Linear programming
• Design of metabolic network
• Constraint based model
• Growth rate
• Production rate
• Smaller reaction network
• Luận án Tiến sĩ
• Physical chemistry
• Fabbricated electrocatalysts
• Oxygen evolution reaction
• Proton exchange membrane
• breast cancer
• medicine
• cytotoxicity
• Atomic nucleus
• electrons
• Chemical compounds
• Electrochemistry