tailieunhanh - Báo cáo khoa học: Choline increases serum insulin in rat when injected intraperitoneally and augments basal and stimulated aceylcholine release from the rat minced pancreas in vitro

Intraperitoneal injection of choline (30–90 mgÆkg )1 ) produced a dose-dependent increase in serum insulin, glu-cose and choline levels in increase in serum insulin induced by choline (90 mgÆkg )1 ) was blocked by pretreat-mentwith themuscarinic acetylcholine receptor antagonists, atropine (2 mgÆkg )1 ), pirenzepine (2 mgÆkg )1 ) and 4-diphe-nylacetoxy-N-methylpiperidine (2 mgÆkg )1 ) or the gangli-onic nicotinic receptor antagonist, hexamethonium (15 mgÆkg )1 ). | Eur. J. Biochem. 270 991-999 2003 FEBS 2003 doi Choline increases serum insulin in rat when injected intraperitoneally and augments basal and stimulated aceylcholine release from the rat minced pancreas in vitro Yesim Ozarda Ilcol1 M. Sibel Gurun2 Yavuz Taga3 and Ismail H. Ulus2 1 Department of Biochemistry and department of Pharmacology and Clinical Pharmacology Uludag University Medical School Bursa Turkey 3Department of Biochemistry Marmara University Medical School Istanbul Turkey Intraperitoneal injection of choline 30-90 mg kg-1 produced a dose-dependent increase in serum insulin glucose and choline levels in rats. The increase in rum insulin induced by choline 90 mg kg-1 was blocked by pretreatment with the muscarinic acetylcholine receptor antagonists atropine 2 mg kg-1 pirenzepine 2 mg kg-1 and 4-diphe-nylacetoxy-V-methylpiperidine 2 mg kg-1 or the ganglionic nicotinic receptor antagonist hexamethonium 15 mg kg-1 . The effect ff diol ire nn eei iim munni mid glucose was enhanced by oral glucose administration 3 g kg-1 . Choline 1111111 11X10011 was associtiled with a significant P increase in the acetylcholine content of pancreatic tissue. Choline 110 330 M increased basal and stimulated acetylcholine release but failed to evoke insulin release from the minced pancreas at considerably higher conedntrations mM . HemicholÌLim-3. a choline uptake inhibitor attenuated the increase in acetylcholine release induced by choline augmentation. Choline 1-32 mM inhibited 3H quinuclidinyl benzilate binding to the muscarinic receptors in the pancreatic homogenates. These data show that choline a precursor of the neurotransmitter acetylcholine increases serum insulin by indirectly stimulating peripheral acetylcholine receptors through the enhancement of acetylcholine synthesis and release. Keywords precursor acetylcholine parasympathetic muscarinic receptors nicotinic receptor. The availability of choline the precursor of the

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