tailieunhanh - Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin

The interaction of heat shock protein with molecular mass 25 kDa (HSP25) and its point mutants S77D + S81D (2D mutant) and S15D + S77D + S81D (3D mutant) with intact and thermally denatured actinwas analyzedbymeans of fluorescence spectroscopy and ultracentrifugation. Wild typeHSP25 did not affect the polymerization of intact actin. The HSP25 3D mutant decreased the initial rate without affecting the maximal extent of intact actin polymerization. G-actin heated at 40–45 C was partially denatured, but retained its ability to polymerize | Eur. J. Biochem. 270 892-901 2003 FEBS 2003 doi Interaction of the small heat shock protein with molecular mass 25 kDa hsp25 with actin Olesya O. Panasenko1 Maria V. Kim1 Steven B. Marston2 and Nikolai B. Gusev1 1 Department of Biochemistry School of Biology Moscow State University Moscow Russia imperial College School of Medicine at National Heart and Lung Institute Dovehose Street London UK The interaction of heat shock protein with molecular mass 25 kDa HSP25 and its point mutants S77D S81D 2D mutant and S15D S77D S81D 3D mutant with intact and thermally denatured actin was analyzed by means of fluorescence spectroscopy and ultracentrifugation. Wild type HSP25 did not affect the polymerization of intact actin. The HSP25 3D mutant decreased the initial rate without affecting the maximal extent of intact actin polymerization. G-actin heated at 40-45 C was partially denatured but retained its ability to polymerize. The wild type HSP25 did not affect polymerization of this partially denatured actin. The 3D mutant of HSP25 increased the initial rate of polymerization of partially denatured actin. Heating at more than 55 C induced complete denaturation of G-actin. Completely denatured G-actin cannot polymerize but it aggregates at increased ionic strength. HSP25 and especially its 2D and 3D mutants effectively prevent salt-induced aggregation of completely denatured actin. It is concluded that the interaction of HSP25 with actin depends on the state of both actin and HSP25. HSP25 predominantly acts as a chaperone and preferentially interacts with thermally unfolded actin preventing the formation of insoluble aggregates. Keywords small heat shock protein actin thermal denaturation. Actin is the major component of the thin filaments of muscle cells and of the cytoskeleton system of nonmuscle cells. It is therefore a very abundant protein and its concentration in smooth muscle is close to 800-900 M 1 . Actin has a rather complex and .

• Báo cáo khoa học
• scientific reports
• Report medicine
• traditional medicine
• Structural biochemistry
• oxidation
• bacteria
• N2 fixation
• Antagonistic effect
• Biological control bacteria pseudomonas
• Inhibits free nitrogen fixing bacteria
• Nitrogen fixing bacteria in the rhizosphere
• Potash mobilizing bacteria
• Rabi sorghum
• Yield of rabi sorghum
• Potash mobilizing bacteria on yield
• Phosphate solubilizing bacteria
• Consortium of azotobacter
• Antibacterial activity of bacteria isolated
• Fermenting cocoa water against
• Pathogenic bacteria
• Selected enteropathogenic bacteria
• Under nails
• Identification of bacteria
• Bacteria from under fingernails
• Identify bacteria contaminants
• IAA producing bacteria
• Indole acetic acid producing bacteria
• Coasts of Ben Tre
• Tra Vinh provinces
• Producing bacteria could naturally stimulate
• Colony bacteria
• Isolation of protease producing bacteria
• Protease producing bacteria
• Soil and characterization
• Characterization of the protease
• Yield parameters
• Phosphate solubilising bacteria
• Potash mobilising bacteria inoculants
• Wheat (Triticum aestivum L
• Inorganic fertilizers on yield of wheat
• Lactic acid bacteria
• Bacteriocin production
• Isolation of probiotic bacteria
• Probiotic bacteria
• Optimization of physical
• Nutrition parameters
• Potassium and phosphorus solubilizing bacteria
• Phosphorus solubilizing bacteria
• Characterization of potassium
• Phosphorus solubilising bacteria
• Employing qualitative plating techniques
• Diazotrophic bacteria
• Influence of diazotrophic bacteria
• Diazotrophic bacteria on growth
• Biomass production
• Sugarcane invitro
• Lactic bacteria
• Physiological parameters
• Food fermented
• Lactic bacteria isolated
• Biodiversity of lactic bacteria isolated
• Lactobacillus sporogenes
• Micrococcus sp
• Histology and intestine
• Lactobacillus sporogenes probiotic bacteria
• Thermophile bacteria
• Growth medium
• Medium growth conditions
• Isolation of thermophilic bacteria
• Thermophilic bacteria
• PhosphateSolubilizing Bacteria (PSB)
• Biochemical characterization
• Rhizosphere soil of rice
• Characterization of phosphate solublizing bacteria
• Phosphate solublizing bacteria
• Lactic acid bacteria and yeast
• Standardization of protocol
• Preparation of linseed beverage
• Cultivable diversity
• Fermented fruit mix
• Characterization of probiotic bacteria
• Isolation of chitinase producing bacteria
• Selection of chitinase producing bacteria
• Antifungal activity against fusarium oxysporum from lilium rhizosphere soil
• Lilium rhizosphere soil
• Chitinase producing bacteria
• Endophytic bacteria
• Bacillus sp
• 16S rRNA sequencing
• Endophytic bacteria from tomato
• Molecular characterization of endophytic bacteria
• Plant Associated Bacteria
• Genomics and recent advances
• The endophytic niche
• Plant growth promoting rhizobacteria
• Their ecology and functions
• Nitrogen fixing bacteria in non legumes
• Water Quality
• Indicator bacteria
• Common sources of E
• coli
• Common routes
• Bacteria to streams
• Risks to human health
• Bacterial diversity
• Sea of Marmara
• Culturable bacteria
• Aerobic heterotrophic bacteria
• Genomic DNAs
• Gram positive bacteria
• Gram negative bacteria
• Lecture AP Biology
• Systems biology
• Biological sciences
• Biological organization
• Isolation of calcite precipitation bacteria
• The strength of concrete
• Materials and methods
• Enrichment of Sample and Bacteria Isolation
• Calcium Carbonate Precipitation and Collection
• Causative bacteria
• Urinary tract infection
• Urinary tract infection in children
• The bacteria was resistant
• The third generation of cephalosporines
• Journal of Automation and Control Engineering
• Development and tuning
• Bacteria foraging optimization algorithm
• Cell formation in cellular manufacturing system
• Cellular manufacturing system
• Bacteria foraging optimization algorithm