tailieunhanh - Báo cáo khoa học: Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix

Minichromosome maintenance proteins (Mcm) 2–7 play essential roles in eukaryotic DNA replication. Several reports have indicated the usefulness of Mcm proteins as markers of cancer cells in histopathological diagnosis. However, their mode of expression and pathophysiological significance in cancer cells remain to be clarified. We com-paredthe level of expressionofMcmproteins amonghuman HeLa uterine cervical carcinoma cells, SV40-transformed human fibroblast GM00637 cells andnormal human fibro-blastWI-38 cells | Eur. J. Biochem. 270 1089-1101 2003 FEBS 2003 doi Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix Yukio Ishimi1. Isao Okavasu2. Chieko Kato1. Hvun-Ju Kwon1 Hiroshi Kimura3 Kouichi Yamada4 and Si-Young Song1 1 Mitsubishi Kagaku Institute of Life Sciences Machida Tokyo department of Pathology Kitasato University School of Medicine Sagamihara Kanagawa 3Department of Functional Genomics Medical Research Institute Tokyo Medical and Dental University Tokyo 4National Institute of Health and Nutrition Tokyo Japan Minichromosome maintenance proteins Mcm 2-7 play essential roles in eukaryotic DNA replication. Several reports have indicated the usefulness of Mcm proteins as markers of cancer cells in histopathological diagnosis. However their mode of expression and pathophysiological significance in cancer cells remain to be clarified. We compared the level of ex pression of Mem pro tei ns among human HeLa uterine cervical carcinoma cells SV40-transformed human fibroblast GM00637 cells and nomial human fibroblast WI-38 cells. All the proteins examined were deteceed in HeLa and GM cells at 6 10 times the eevel I ound in WI-88 cells on average. This increase was observed both m total cellular proteins andin the chromatin-boundfraction. Consistently Mcm2 mRNA was enriched m HeLa ceUs to approximately four times the level in WI-38 cells andthe synthesis of Mcm4 6 and 7 lorten s was accdera ted ìn He La cells. Immunohistochemical studies of surgical materials from human uterine cervix showedthat Mcm3 and4 are ubiquitously expressed m cancer ceUs. l u r th r. the oOii iv e rate andlevel of Mcm3 and4 expression appearedto be higher in cancer cells than in normal proliferating cells of the uterine cervix anddysplastic cells suggesting that they can be useful markers to distinguish these cells. Keywords cancer cells DNA replication Mcm protein expression uterine cervix. The entire Mcm family Mcm2-7 is essential for .

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