tailieunhanh - Báo cáo khoa học: Deciphering metabolic networks

All higher organisms divide major biochemical steps into different cellular compartments and often use tissue-specific division of metabolism for the same spatial resolution is accompanied with temporal changes of meta-bolite synthesis in response to environmental stimuli or developmental analyses of primary and secondary gene products, . transcripts, proteins, and metabolites, regularly do not cope with this spatial and temporal resolution, these gene products are often observed to be highly coregulated forming complex networks | Eur. J. Biochem. 270 579-588 2003 FEBS 2003 doi MINIREVIEW Deciphering metabolic networks Oliver Fiehn and Wolfram Weckwerth Max-Planck Institute of Molecular Plant Physiology 14424 PotsdamịGolm Germany All higher organisms divide major biochemical steps into different cellular compartments and often use tissue-specific division of metabolism for the same purpose. Such spatial resolution is accompanied with temporal changes of metabolite synthesis in response to environmental stimuli or developmental needs. Althoghh analyses of primary add secondary gene products . transcripts proteins and metabolites regularly do not cope with this spatial and temporal resolution these gene products are often observed to be highly coregulated forming complex networks. Me hinds to tlLidy sucIi lsetwores lire eevewved with respect to data acquisition network statistics and biochemical interpretation. Keywords metabolomics proteomics protein networks metabolite networks metabolite profiling. Introduction Now that a variety of unicellular and multicellular genomes have successfully been sequenced and partially annotated see The Arabidopsis Genome Initiative 2000 1 2 functional genomics has become a focal point for many research efforts. For mstl sanorees a inli 11 1 1111 number ơi gness cannot be annotated by homology to genes in other organisms and with the exception for yeast for the majority of the annotated genes no experimental proof is supporting these annotations. Molhwer. if fonusing on metabolism many levels of regulation occur after genes have been transcribed such as post-transcriptional translational post-translational and all forms of biochemical control such as allosteric or feedback this view into account it is hard to believe that functional genomics can stop at the mRNA level. Istrend. tem biology approaches need to be undertaken that comprehensively analyse the structure of cellular organization and try to model .