tailieunhanh - Báo cáo khoa học: Proteome analysis at the level of subcellular structures Mathias Dreger

The targeting of proteins to particular subcellular sites is an important principle of the functional organization of cells at themolecular level. In turn, knowledge about the subcellular localizationof aprotein is acharacteristic thatmayprovidea hint as to the function of the protein. The combination of classic biochemical fractionation techniques for the enrich-ment of particular subcellular structures with the large-scale identification of proteins by mass spectrometry and bio-informatics provides a powerful strategy that interfaces cell biology andproteomics, andthus is termed subcellular proteomics . . | Eur. J. Biochem. 270 589-599 2003 FEBS 2003 doi MINIREVIEW Proteome analysis at the level of subcellular structures Mathias Dreger Institute for Chemistry Biochemistry Free University Berlin Germany The targeting of proteins to particular subcellular sites is an important principle of the functional organization of cells at the molecular level. In turn knowledge about the subcellular localization of a protein is a characteristic that may provide a hint as to the function of the protein. The combination of classic biochemical fractionation techniques for the enrichment of particular subcellular structures with the large-scale identification of proteins by mass spectrometry and bioinformatics provides a powerful strategy that interfaces cell biology and proteomics add thus is termed subcellular proteomics . In addition to its exceptional power for the identification of previously unknown gene products the analysis of proteins at the subcellular level is the basis for monitoring important aspects of dynamic changes in the proteome such as protein transloction. This review summarizes data from recent subcellular proteomics studies with an emphasis on the type of data that can retrieved from such studies depending on the design of the analytical strategy. Keywords subcellular proteomics mass spectrometry organelle synapse nucleus membrane protein functional genomics. Introduction With the increasing degree of complexity organisms acquire a broader repertoire of options to meet enviromental challenges. This increasedcomplexity of organisms is realized at two leel ls irstl ly not all fells of the oi annism serve the same purpose the organism contains several different subsets of cells with distinct properties for example neurons germ cells or epithelial cells. Secondly within a given cell functions such as storage of genetic material degradation of proteins or the provision of energy-rich metabolites to fuel cellular reactions are .