tailieunhanh - Báo cáo khoa học: Antioxidant protein 2 prevents methemoglobin formation in erythrocyte hemolysates

Antioxidant protein 2 (AOP2) is a member of a family of thiol-specific antioxidants, recently renamedperoxiredoxins, that evolvedas part of anelaborate systemtocounteract and control detrimental effects of oxygen radicals. AOP2 is found in endothelial cells, erythrocytes, monocytes, T andB cells, but not in granulocytes. AOP2 was found solely in the cytoplasm and was not associated with the nuclear or membrane fractions; neither was it detectable in plasma. Further experiments focused on the function of AOP2 in erythrocytes where it is closely associated with the hemo-globin complex, particularlywith theheme | Eur. J. Biochem. 270 334-341 2003 FEBS 2003 doi Antioxidant protein 2 prevents methemoglobin formation in erythrocyte hemolysates Karl M. Stuhlmeier1 Janet J. Kao2 Pia Wallbrandt3 Maria Lindberg3 Barbro Hammarstrom3 Hans Broell1 and Beverly Paigen4 1 Ludwig Boltzmann Institute for Rheumatology and Balneology Vienna Austria 2Maimonides Medical Center Brooklyn NY USA 3Department of Molecular Biology AstraZeneca Umeâ Sweden and 4The Jackson Laboratory Bar Harbor ME USA Antioxidant protein 2 AOP2 is a member of a family of thiol-specific antioxidants recently renamed peroxiredoxins that evolved as part of an elaborate system to counteract and control detrimental effects of oxygen radicals. AOP2 is found in endothelial cells erythrocytes monocytes T and B cells but not in granulocytes. AOP2 was found solely in the cytoplasm and was not associated with the nuclear or membrane fractions neither was it detectable in plasma. Further experiments focused on the function of AOP2 in erythrocytes where it is closely associated with the hemoglobin complex particularly with the heme. An investigation of the mechanism of this interaction demonstrated that the conserved cysteine-47 in AOP2 seems to play a role in AOP2-heme interactions. Recombinant AOP2 prevented induced as well as noninduced methemoglobin formation in erythrocyte hemolysates indicating its antioxidant properties. We conclude that AOP2 is part of a sophisticated system developed to protect and support erythrocytes in their many physiological functions. Keywords hemoglobin erythrocytes reactive oxygen species antioxidant protein 2. Evolving antioxidant defence systems to protect against O2 toxicity has been a prerequisite for an organism s use of O2 for efficient energy production. To benefit from O2 as an energy source multicellular organisms had to develop a system to distribute O2. In mammals this function is carried out by red blood cells RBC which utilize hemoglobin to distribute

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