tailieunhanh - Báo cáo khoa học: Expression of MsPG3-GFP fusions in Medicago truncatula Ôhairy rootsÕ reveals preferential tip localization of the protein in root hairs

Tip growth is a specialized type of polar growth where new cell wall is deposited in a localized region of the cell, the growing tip. These cells show a characteristic zonation, with a high accumulationof secretory vesicles containing cell wall components at the tip, followed by an organelle-enriched a Medicago sativapolygalacturonase gene isolated in our laboratory, specifically expressed during the interaction of this plant with its symbiotic partner Sinorhiz-obium melilotiand which might participate in tip growth processes during symbiosis | Eur. J. Biochem. 270 261-269 2003 FEBS 2003 doi Expression of MsPG3-GFP fusions in Medicago truncatula hairy roots reveals preferential tip localization of the protein in root hairs Ignacio D. Rodriguez-Llorente1 Javier Perez-Hormaeche1 Mohammed Dary1 Miguel A. Caviedes1 Adam Kondorosi2 3 Pascal Ratet2 and Antonio J. Palomares1 1 Departamento de Microbiologia y Parasitologia Facultad de Farmacia Universidad de Sevilla Spain 2Institut des Sciences Végétales Centre National de la Recherche Scientifique Gif sur Yvette France 3Institute of Genetics Biological Research Center Hungarian Academy of Sciences Szeged Hungary Tip growth is a specialized type of polar growth where new cell wall is deposited in a localized region of the cell the growing tip. These cells show a characteristic zonation with a high accumulation of secretory vesicles containing cell wall components at the tip followed by an organelle-enriched zone. MsPG3 is a Medicago sativa polygalacturonase gene isolated in our laboratory specifically expressed during the interaction of this plant with its symbiotic partner Sinorhiz-obium meliloti and which might participate in tip growth processes during symbiosis. We have used MsPG3-GFP fusions to study in vivo protein transport processes and localization during root hair growth. Different MsPG3-GFP fusions were expressed in Medicago truncatula hairy roots following a protocol developed for this study and also tested by transient expression in onion epidermal cells. Preferential accumulation of an MsPG3-GFP fusion protein in the tip of the growing root hair at different developmental stages was found confirming the delivery of MsPG3 to the newly synthesized cell wall. This indicates that this protein may participate in tip growth processes during symbiosis and in addition that this fusion could be a useful tool to study this process in plants. Keywords GFP hairy root Medicago truncatula polygalacturonase tip growth. Plant cells