tailieunhanh - Báo cáo khoa học: The effect of ST2 gene product on anchorage-independent growth of a glioblastoma cell line, T98G

TheST2gene, which is specifically induced by growth sti-mulation in fibroblasts, encodes interleukin-1 receptor-rela-tedproteins and iswidely expressed in hematopoietic, helper T, and various cancer cells. However, the physiological as well as pathological functions of the ST2 gene products are not yet fully understood. In this study, we analyzed the expression of theST2gene in human glioma cell lines and human brain tumor samples with real-time polymerase chain reactionmethod, | Eur. J. Biochem. 270 163-170 2003 FEBS 3 oi j M 2-10 22 J ji The effect of ST2 gene product on anchorage-independent growth of a glioblastoma cell line T98G Yasushi Haga1 Ken Yanagisawa2 Hiromi Ohto-Ozaki2 Shin-ichi Tominaga2 Toshio Masuzawa1 and Hiroyuki Iwahana2 1 Department of Surgical Neurology and department of Biochemistry Jichi Medical School Minamikawachi-machi Kawachi-gun Tochigi Japan The ST2 gene which is specifically induced by growth stimulation in fibroblasts encodes interleukin-1 receptor-related proteins and is widely expressed in hematopoietic helper T and various cancer cells. However the physiological as well as pathological functions of the ST2 gene products are not yet fully understood. In this study we analyzed the expression of the ST2 gene in human glioma cell lines and human brain tumor samples with real-time polymerase chain reaction method the results of which revealed that the expression level of the ST2 gene in glioma cell lines and glioblastoma samples is significantly lower than that in a fibroblastic cell line TM12 and benign brain tumors suggesting the reverse relationship between malignancy and ST2 expression. As we could not detect the soluble ST2 protein in the culture fluid of the T98G glioblastic cell line by ELISA we established stable transformants of T98G that continuously produce and secrete the ST2 protein in order to study the effect of the ST2 protein on malignancy. Although we could not detect a remarkable difference in proliferation between transformants and control cells in conventional tissue culture dishes the efficiency of colony formation in soft agar was significantly decreased in the case of cells that continuously produce the ST2 protein. Furthermore inhibition of colony formation in soft agar was observed in wild-type T98G cells when purified soluble ST2 protein was added to the culture in a dose-dependent manner. Taken together the results suggest that the expression of ST2 suppressed the .

• Báo cáo khoa học
• scientific reports
• Report medicine
• traditional medicine
• medical research
• hemoglobin
• medical knowledge
• medical textbooks
• medical practice
• specialized medicine
• Encyclopedia of Medical Anthropology
• medical
• health
• medical term dictionary
• y tế
• sức khỏe
• Data Mining and Medical Knowledge Management
• Premier Reference Sourse
• statements and documents scientific reports
• medical reports
• medical transfer
• the developing countries
• clinical trials
• medical management
• health care
• Healthcare knowledge management
• Knowledge identification
• Know How and Knowing That cartography
• Knowledge visualization
• Improving knowledge management
• Knowledge workers’ identities
• Evidence from the medical profession
• Knowledge workers
• Medical profession
• Organizational identification
• Knowledge Management
• Data Mining
• Text Mining
• Mapping Medical
• Bioinformatics Challenges
• Digital Libraries
• Medical Informatics
• A Handbook for Medical Teachers
• Medical Teachers
• health education
• health knowledge