tailieunhanh - Báo cáo khoa học: Structure of the O polysaccharides and serological classification of Pseudomonas syringae pv. porri from genomospecies 4

Strains ofPseudomonas syringaepv. porri are characterized by a number of pathovar-specific phenotypic and genomic characters and constitute a highly homogeneous group. Using monoclonal antibodies, they all were classified in a novelP. syringaeserogroup O9. The O polysaccharides (OPS) isolated from the lipopolysaccharides (LPS) of P. syringaepv. porri NCPPB 3365 and NCPPB 3364 T possess multiple oligosaccharide O repeats, some of which are linear and composed ofL-rhamnose (L-Rha), whereas the major O repeats are branched with L-rhamnose in the main chain and GlcNAc in side chains (structures1and2). Both branched O repeats, which differ in the position of substitution of one of the Rha residues and in the site of attachment of GlcNAc, were found in the. | Eur. J. Biochem. 270 20-27 2003 FEBS 2003 doi Structure of the O polysaccharides and serological classification of Pseudomonas syringae pv. porri from genomospecies 4 Evelina L. Zdorovenko1 George V. Zatonskii1 Nina A. Kocharova1 Aleksander S. Shashkov1 Yuriy A. Knirel1 and Vladimir V. Ovod2 1N. D. Zelinsky Institute of Organic Chemistry Russian Academy of Sciences Moscow Russia 2Institute of Medical Technology University of Tampere Tampere Finland Strains of Pseudomonas syringae pv. porri are characterized by a number of pathovar-specific phenotypic and genomic characters and constitute a highly homogeneous group. Using monoclonal antibodies they all were classified in a attachment of GlcNAc were found in the two strains studied O repeat 1 being major in strain NCPPB 3365 and 2 in strain nCpPB 3364t. fi3 -a-L-Rhap- 1 fi2 -a-L-Rhap- 1 fi3 -a-L-Rhap- 1 fi3 -a-L-Rhap- 1 fi 1 2 1 b-D-GlcpNAc fi2 -a-L-Rhap- 1 fi2 -a-L-Rhap- 1 fi3 -a-L-Rhap- 1 fi3 -a-L-Rhap- 1 fi 2 2 1 b-D-GlcpNAc novel P. syringae serogroup O9. The O polysaccharides OPS isolated from the lipopolysaccharides LPS of P. syringae pv. porri NCPPB 3365 and NCPPB 3364T possess multiple oligosaccharide O repeats some of which are linear and composed of L-rhamnose L-Rha whereas the major O repeats are branched with L-rhamnose in the main chain and GlcNAc in side chains structures 1 and 2 . Both branched O repeats which differ in the position of substitution of one of the Rha residues and in the site of The relationship between OPS chemotype and serotype on one hand and the genomic characters of P. syringae pv. porri and other pathovars delineated in genomospecies 4 on the other hand is discussed. Keywords lipopolysaccharide O polysaccharide structure serological classification monoclonal antibody Pseudomonas syringae. Strains of the phytopathogenic bacterium Pseudomonas syringae are characterized by a high degree of heterogeneity in respect to phenotypic and genotypic .