tailieunhanh - Báo cáo khoa học: The activation of gelsolin by low pH The calcium latch is sensitive to calcium but not pH

Gelsolin is a multidomain and multifunction protein that nucleates the assembly of filaments and severs them. The activation of gelsolin by calcium is a multistep process involving many calcium binding sites that act to unfold the molecule from a tight structure to a more loose form in which three actin-binding sites become exposed. Low pHis alsoknowntoactivategelsolin, in theabsenceof calciumand this too results in an unfolding of the molecule. | Eur. J. Biochem. 270 4105-4112 2003 FEBS 2003 doi The activation of gelsolin by low pH The calcium latch is sensitive to calcium but not pH Emeline Lagarrigue1 Diane Ternent2 Sutherland K. Maciver2 Abdellatif Fattoum3 Yves Benyamin1 and Claude Roustan1 1UMR 5539 CNRS Laboratoire de motilité cellulaire Ecole Pratique des Hautes Etudes Université de Montpellier 2 Montpellier Cedex 5 France 2Genes and Development Group Department of Biomedical Sciences University of Edinburgh Hugh Robson Building George Square Edinburgh Scotland 3Centre de Recherches de Biochimie Macromoleculaire UPR 1086 CNRS Montpellier Cedex 5 France Gelsolin is a multidomain and multifunction protein that nucleates the assembly of filaments and severs them. The activation of gelsolin by calcium is a multistep process involving many calcium binding sites that act to unfold the molecule from a tight structure to a more loose form in which three actin-binding sites become exposed. Low pH is also known to activate gelsolin in the absence of calcium and this too results in an unfolding of the molecule. Less is known how pH-activation occurs but we show that there are significant differences in the mechanisms that lead to activation. Crucially while it is known that the bonds between G2 and G6 are broken by co-operative occupancy of calcium binding sites in both domains Lagarrique E. Maciver S. K. Fattoum A. Benyamin Y. Roustan C. 2003 Eur. J. Biochem. 270 2236-2243. pH values that aciivate gelsolin do not result in a weakening of the G2-G6 bonds. We report the existence of pH-dependent conformational changes within G2 and in G4-6 that differ from those induced by calcium and that low pHoverrides the requirement for calcium for actin-binding within G4-6 to a modest extent so that a Kd of 1 IM is measured compared to 30-40 nM in the presence of calcium. Whereas the pH-dependent conformational change in G2 is possibly different from the change induced by calcium the .

• Báo cáo khoa học
• scientific reports
• Report medicine
• traditional medicine
• oxidation
• Crystal structure
• bacteria
• N2 fixation
• Antagonistic effect
• Biological control bacteria pseudomonas
• Inhibits free nitrogen fixing bacteria
• Nitrogen fixing bacteria in the rhizosphere
• Potash mobilizing bacteria
• Rabi sorghum
• Yield of rabi sorghum
• Potash mobilizing bacteria on yield
• Phosphate solubilizing bacteria
• Consortium of azotobacter
• Antibacterial activity of bacteria isolated
• Fermenting cocoa water against
• Pathogenic bacteria
• Selected enteropathogenic bacteria
• Under nails
• Identification of bacteria
• Bacteria from under fingernails
• Identify bacteria contaminants
• IAA producing bacteria
• Indole acetic acid producing bacteria
• Coasts of Ben Tre
• Tra Vinh provinces
• Producing bacteria could naturally stimulate
• Colony bacteria
• Isolation of protease producing bacteria
• Protease producing bacteria
• Soil and characterization
• Characterization of the protease
• Yield parameters
• Phosphate solubilising bacteria
• Potash mobilising bacteria inoculants
• Wheat (Triticum aestivum L
• Inorganic fertilizers on yield of wheat
• Lactic acid bacteria
• Bacteriocin production
• Isolation of probiotic bacteria
• Probiotic bacteria
• Optimization of physical
• Nutrition parameters
• Potassium and phosphorus solubilizing bacteria
• Phosphorus solubilizing bacteria
• Characterization of potassium
• Phosphorus solubilising bacteria
• Employing qualitative plating techniques
• Diazotrophic bacteria
• Influence of diazotrophic bacteria
• Diazotrophic bacteria on growth
• Biomass production
• Sugarcane invitro
• Lactic bacteria
• Physiological parameters
• Food fermented
• Lactic bacteria isolated
• Biodiversity of lactic bacteria isolated
• Lactobacillus sporogenes
• Micrococcus sp
• Histology and intestine
• Lactobacillus sporogenes probiotic bacteria
• Thermophile bacteria
• Growth medium
• Medium growth conditions
• Isolation of thermophilic bacteria
• Thermophilic bacteria
• PhosphateSolubilizing Bacteria (PSB)
• Biochemical characterization
• Rhizosphere soil of rice
• Characterization of phosphate solublizing bacteria
• Phosphate solublizing bacteria
• Lactic acid bacteria and yeast
• Standardization of protocol
• Preparation of linseed beverage
• Cultivable diversity
• Fermented fruit mix
• Characterization of probiotic bacteria
• Isolation of chitinase producing bacteria
• Selection of chitinase producing bacteria
• Antifungal activity against fusarium oxysporum from lilium rhizosphere soil
• Lilium rhizosphere soil
• Chitinase producing bacteria
• Endophytic bacteria
• Bacillus sp
• 16S rRNA sequencing
• Endophytic bacteria from tomato
• Molecular characterization of endophytic bacteria
• Plant Associated Bacteria
• Genomics and recent advances
• The endophytic niche
• Plant growth promoting rhizobacteria
• Their ecology and functions
• Nitrogen fixing bacteria in non legumes
• Water Quality
• Indicator bacteria
• Common sources of E
• coli
• Common routes
• Bacteria to streams
• Risks to human health
• Bacterial diversity
• Sea of Marmara
• Culturable bacteria
• Aerobic heterotrophic bacteria
• Genomic DNAs
• Gram positive bacteria
• Gram negative bacteria
• Lecture AP Biology
• Systems biology
• Biological sciences
• Biological organization
• Isolation of calcite precipitation bacteria
• The strength of concrete
• Materials and methods
• Enrichment of Sample and Bacteria Isolation
• Calcium Carbonate Precipitation and Collection
• Causative bacteria
• Urinary tract infection
• Urinary tract infection in children
• The bacteria was resistant
• The third generation of cephalosporines
• Journal of Automation and Control Engineering
• Development and tuning
• Bacteria foraging optimization algorithm
• Cell formation in cellular manufacturing system
• Cellular manufacturing system
• Bacteria foraging optimization algorithm