tailieunhanh - Báo cáo khoa học: Lysosomal enzymes promote mitochondrial oxidant production, cytochrome c release and apoptosis

Exposure of mammalian cells to oxidant stress causes early (iron catalysed) lysosomal rupture followed by apoptosis or necrosis. Enhanced intracellular production of reactive oxygen species (ROS), presumably of mitochondrial origin, is also observed when cells are exposed to nonoxidant pro-apoptotic agonists of cell death. We hypothesized that ROS generation in this latter case might promote the apoptotic cascade and could arise from effects of released lysosomal materials on mitochondria. | Eur. J. Biochem. 270 3778-3786 2003 FEBS 2003 doi j Lysosomal enzymes promote mitochondrial oxidant production cytochrome c release and apoptosis Ming Zhao1 Fernando Antunes1 2 John W. Eaton1 3 and Ulf T. Brunk1 - Division of Pathology II Faculty of Health Sciences Linkoping University Sweden 2Grupo de Bioquimica e Biologia Teoricas -Instituto Bento da Rocha Cabral and Department of Chemistry and Biochemistry Faculty of Sciences University of Lisbon Portugal 3 James Graham Brown Cancer Center University of Louisville Louisville KY USA Exposure of mammalian cells to oxidant stress causes early iron catalysed lysosomal rupture followed by apoptosis or necrosis. Enhanced intracellular production of reactive oxygen species ROS presumably of mitochondrial origin is also observed when cells are exposed to nonoxidant pro-apoptotic agonists of cell death. We hypothesized that ROS generation in this latter case might promote the apoptotic cascade and could arise from effects of released lysosomal materials on mitochondria. Indeed in intact cells J774 macrophages HeLa cells and AG-5-8 fibroblasts the lysosomotropic detergent O-methyl-serine dodecylamide hydrochloride MSDH causes lysosomal rupture enhanced intracellular ROS production and apoptosi j. Futthermore. in mixtures of rat liver lysosomes and mitochondria selective rupture of lysosomes by MSDH promotes mitochondrial ROS prodLiciíon and cytochrome c release whereas MSDH has no direct effect on ROSgeneration by purifed mito chondria. Intracellular lysosomal rupture is associated with the release of among other constituents cathepsins and activation of phospholipase A2 PLA2 . We find that addition of purified cathepsins B or D or of PLA2 causes substantial increases in ROSgeneration by purified mitochondria. Furthermore PLA2 - but not cathepsins B or D - causes rupture of semipurified lysosomes suggesting an amplification mechanism. Thus initiation of the apoptotic cascade by nonoxidant .

• Báo cáo khoa học
• scientific reports
• Report medicine
• traditional medicine
• tumor cells
• medical knowledge
• chemical reaction
• Elements of Chemical Reaction Engineering
• Chemical Reaction Engineering
• Hóa học và ứng dụng
• Kỹ thuật hóa học
• Phản ứng hóa học
• Kỹ thuật phản ứng hóa học
• The chemical vapor deposition condition
• The electrochemically catalytic efficiency
• Hydrogen evolution reaction in MoS2 nanoparticles
• Hydrogen evolution reaction
• Metal organic chemical vapor deposition
• Reaction Kinetics
• Heterogeneous Catalysis
• Elementary Reactions
• Microkinetic Analysis
• Nonisothermal Reactors
• Catalytic Reactions
• Quantum chemical modeling mechanism
• Semiempirical method RM1
• Quantum chemical study
• Half chair shape
• Diels Alder reaction
• Chemical reaction engirneering
• Hoá phân tích
• Giải pháp phản ứng hóa học
• Radiation on MHD casson fluid flow
• Chemical reaction effects on MHD casson fluid flow
• Porous medium with suction injection
• The occurrence of suction/injection
• The central PDEs
• Radiation of MHD visco elastic fluid flow over
• Chemical reaction on heat of MHD visco elastic
• Mass transfer of MHD visco elastic fluid flow
• Exponentially stretching sheet through porous medium
• Radiation on the boundary layer MHD casson fluid
• Chemical reaction effects on the boundary layer MHD casson fluid
• Vertical plate embedded in the porous medium
• Casson fluid flow free convection boundary layer fl
• Extreme learning machine
• Artificial chemical reaction optimization algorithm
• Single hidden layer feed forward neural network
• Learning algorithm
• Chemical reaction kinetic
• Adsorption kinetics
• Pseudo first order model
• Pseudo second order model
• General kinetic model
• Dried Probiotics
• Industrial Processes
• Membrane Separation
• Wastewater
• Maillard Reaction
• Pros and Cons
• Chemical kinetics
• Reactant concentration
• Reaction rates and stoichiometry
• Action of catalysts
• Surface area
• Chemical kinetics
• Introduction to general
• Chemical bonds
• Chemical reactions
• Reaction rates
• Chemical equilibrium
• Nuclear chemistry
• Nuclear engineering and technology
• Correlation between rare earth elements
• The chemical interactions of HT9 cladding
• FueleCladding Chemical Interaction
• Higher reaction thickness
• Summary of Chemical PhD Thesis
• Theoretical and Physical Chemistry
• CO hydrogenation reaction
• Ni Cu bimetallic catalyst system
• Density functional method
• Chemical method
• Lecture General Chemistry 1
• Bài giảng Hóa học đại cương 1
• Balance chemical equations
• Calculate the percent yield of a reaction
• Chemical formulas
• Chemical equations
• organic chemistry
• hóa hữu cơ
• công nghệ hóa học
• Chemical Engineering
• common reaction mechanisms
• Chromatography in the petroleum industry
• oxidation
• BMC Bioinformatics
• Flux balance analysis
• Linear programming
• Design of metabolic network
• Constraint based model
• Growth rate
• Production rate
• Smaller reaction network
• Luận án Tiến sĩ
• Physical chemistry
• Fabbricated electrocatalysts
• Oxygen evolution reaction
• Proton exchange membrane
• breast cancer
• medicine
• cytotoxicity
• Atomic nucleus
• electrons
• Chemical compounds
• Electrochemistry